Integrative analyses of the kinetochore and the spindle assembly checkpoint

摘要 我的实验室将细胞生物学和生物物理学结合起来，对两个复合体进行综合分析 细胞系统：着丝粒，一种在有丝分裂期间移动染色体的蛋白质机器， 和主轴组件检查点(SAC)，这是一个细胞周期控制。即使我们正在制作 在我们项目的既定目标方面取得了很好的进展，我已经开始开发下一阶段的 这项研究涉及用从头开始取代动粒蛋白质机器的组件 设计的蛋白质。为了这项激动人心的工作，我聘请了马丁·费尔南德斯先生，他是一名新近毕业的学生 来自利哈伊大学。马丁在我的实验室担任研究技术员，他花了60%的时间 积极研究的时间，包括基准工作、数据分析和演示，以及剩余的时间 40%的时间用于实验室管理职责。我有幸与马丁一起工作了 6个多月，这一经历让我清楚地知道：(A)马丁拥有 致力于成为生物医学科学的专业人士，以及(B)马丁有激情 从事基础研究，并希望最终加入学术界。根据我的经验，我是 他有信心在来年成功申请参加研究生课程。 因此，我希望通过确保马丁在我的实验室的工作对他的职业生涯产生最大影响 即：(A)他将100%的时间用于研究；(B)他充分受益于 我能提供的指导和发展资源。因此，我和马丁一起， 我正在申请一份多样性补助金，以支付马丁一年的努力。在这一年，马丁 我基本上是作为我实验室的一名研究生工作，并推动一项令人兴奋的研究项目 往前走。他将学习尖端的实验方法(负染色EM显微镜，单 分子成像等)。他还将从事职业发展活动(学习 使用Python编写代码，与我所在机构的研究生和教职员工建立联系，介绍他的 在ASCB会议上工作，整理一份精致的研究生院申请，等等。我是 相信马丁将充分利用随之而来的财政支持和认可 并在不久的将来开始他的科学家生涯。

Abstract My lab combines cell biology and biophysics to conduct integrative analyses of two complex cellular systems: the kinetochore, a protein machine that moves chromosomes during mitosis, and the Spindle Assembly Checkpoint (SAC), which is a cell cycle control. Even as we are making excellent progress on the stated aims of our project, I have started developing the next phase of this research that involves replacing components of the kinetochore protein machine with de novo designed proteins. For this exciting work, I recruited Mr. Martin Fernandez, a recent graduate from the Lehigh University. Martin works in my lab as a research technician, spending 60% of his time on active research involving benchwork, data analysis, and presentation, and the remaining 40% of the time on lab management duties. I have had the pleasure of working with Martin for over 6 months, and this experience makes it clear to me that: (a) Martin possesses the talent and dedication to emerge as a professional in the biomedical sciences, and (b) Martin has a passion for basic research and wants to ultimately join the academia. Based on my experience, I am confident that he will successfully apply for joining a graduate program in the coming year. Therefore, I want to maximize the impact of Martin’s work in my lab over his career by ensuring that: (a) he dedicates 100% of his time to research, and (b) he gets the full benefit of the mentorship and development resources that I can provide. For this reason, I, together with Martin, am applying for a diversity supplement to cover Martin’s efforts for 1 year. During this year, Martin will work essentially as a new graduate student in my lab and drive an exciting research project forward. He will learn cutting-edge experimental methods (negative-stain EM microscopy, single molecule imaging, etc.). He will also engage in professional development activities (learning to code using Python, networking with graduate students and faculty at my institution, presenting his work at the ASCB meeting, putting together a polished application for graduate school, etc.). I am confident that Martin will fully utilize the financial support and recognition that comes with this funding and start his career as a scientist in the near future.