Direct therapeutic intervention of the tumor microenvironment with a potent inhibitor of fibronectin assembly

项目摘要 在癌症的标志中，细胞外基质有助于调节每个标志， 肿瘤进展的基本原则。重要的是，细胞外基质硬度和纤维组织 增加乳腺癌的整体进展，并与患者预后不良相关。生物物理和 来自纤维状基质硬度的生物化学线索已经成为转移性肿瘤发生步骤的关键调节因子。 级联反应，包括增加的肿瘤细胞侵袭、炎性信号传导、循环肿瘤细胞和转移性肿瘤细胞。 结果此外，新兴的研究表明，来自ECM的生物物理线索影响肿瘤内在的细胞因子。 以及与免疫疗法抗性有关的外在因素。总之，这些数据表明，基质 基质硬度可能是乳腺肿瘤免疫抑制的潜在机制之一 微环境尽管越来越多的证据表明生物物理学线索在疾病进展中起着关键作用 以及对治疗靶向肿瘤ECM的兴趣越来越大，还没有直接有效的治疗方法 靶向乳腺癌中的基质。因此，我们建议开发一种治疗上有用的药剂， 直接破坏原发性和转移性肿瘤内的细胞外基质组装、沉积和组织， 乳腺癌的临床治疗的肿瘤微环境。我们假设直接瞄准 FN与PEG化FUD的组装随后将通过抑制胶原蛋白而减少肿瘤纤维化 沉积和纤维排列导致肿瘤生长和转移进展减少。我们 进一步假设减少的纤连蛋白和胶原沉积将限制促肿瘤的机械活化 炎症导致与免疫检查点阻断组合的增强的治疗功效。我们 目的1：确定PEG化FUD作为抗癌药物的功效 乳腺癌的临床前模型的治疗。目标2.评价聚乙二醇化FUD对 免疫抑制信号传导以增强用于治疗转移性疾病的抗PD-L1疗法。

PROJECT SUMMARY Among the hallmarks of cancer, the extracellular matrix contributes to the regulation of each of the hallmark principles underlying tumor progression. Importantly, extracellular matrix stiffness and fiber organization enhance overall breast cancer progression and are associated with poor patient outcome. Biophysical and biochemical cues from fibrillar matrix stiffness have emerged as key regulators of steps in the metastatic cascade, including increased tumor cell invasion, inflammatory signaling, circulating tumor cells, and metastatic outgrowth. Moreover, emerging studies demonstrate that biophysical cues from the ECM impact tumor intrinsic and extrinsic factors implicated in immunotherapy resistance. Taken together, this data suggests that stromal matrix stiffness may be one of the underlying mechanisms driving immunosuppression in the breast tumor microenvironment. Despite the growing evidence that biophysical cues play a key role in disease progression and the mounting interest in therapeutically targeting tumor ECM, there has yet to be an effective therapy directly targeting the stromal matrix in breast cancer. Therefore, we propose to develop a therapeutically useful agent to directly disrupt extracellular matrix assembly, deposition, and organization within the primary and metastatic tumor microenvironments for the clinical treatment of breast cancer. We hypothesize that directly targeting FN assembly with PEGylated-FUD will subsequently reduce tumor fibrosis by inhibiting collagen deposition and fiber alignment resulting in decreased tumor growth and metastatic progression. We further hypothesize that reduced fibronectin and collagen deposition will limit mechanical activation of pro-tumor inflammation resulting in enhanced therapeutic efficacy in combination with immune checkpoint blockade. We will test our hypothesis in the following aims: Aim 1: Determine the efficacy of PEGylated FUD as an anti-cancer therapy in pre-clinical models of breast cancer. Aim 2. Evaluate the impact of PEGylated-FUD on immunosuppressive signaling to enhance anti-PD-L1 therapy for treatment of metastatic disease.