High Speed, High Resolution Slide Scanner for Research in Translational Medicine

项目摘要/摘要 本申请是阿尔伯特·爱因斯坦的分析成像设备(AIF)提供的仪器资助 医学院将收购一款全自动、高容量、高分辨率的幻灯片扫描仪，该扫描仪可以 同时适应明场成像和荧光成像。阿尔伯特·爱因斯坦医学院AIF 通过让NIH资助的研究人员获得最先进的显微技术来支持他们 加强协作、多学科研究。收购这一工具将对 在爱因斯坦进行生物医学研究，并将扩大NIH资助的爱因斯坦项目的范围。几个项目 已经确定将大量使用扫描仪。其中包括：入侵形成的研究 用于在癌细胞中系统地扩散转移癌细胞的促进结构(Condeelis)， 相关活体组织病理学成像解剖肿瘤微环境(Entenberg)，建立 和免疫受损放射性直肠炎小鼠模型(GUHA)的验证，研究转移性 乳腺癌(Karagiannis)新辅助化疗的疗效观察 环境对转移的影响(Oktay)，后续乳腺侵袭性风险的分子标志物分析 女性导管原位癌(Rohan)与肝细胞异质性的研究 基因转录特征及其在健康组织完整肝脏内的带状分布 疾病患者组织(歌手)。在爱因斯坦中也有额外的小用户和多用户的项目 社区是可以期待的。这个应用中的所有项目都需要高容量和高性能的 可重复性扫描，以便分析可以应用于覆盖数百个视场的较大组织， 而不是在标准显微镜上获得的单一视场。

PROJECT SUMMARY/ABSTRACT This application is an instrumentation grant from the Analytical Imaging Facility (AIF) at the Albert Einstein College of Medicine to acquire a fully automated, high-capacity, high-resolution slide scanner that can accommodate both brightfield and fluorescence imaging. The AIF of the Albert Einstein College of Medicine supports NIH-funded investigators by giving them access to state-of-the-art microscopy technologies that enhance collaborative, multidisciplinary research. Acquisition of this instrument will have a high impact on the biomedical research at Einstein and will expand the scope of NIH-funded projects at Einstein. Several projects have been identified that will heavily utilize the scanner. These include: the study of the formation of invasion promoting structures used to systemically disseminate metastatic cancer cells in carcinoma (Condeelis), correlative Intravital-Histopathologic Imaging to dissect the tumor microenvironment (Entenberg), establishment and validation of an immunocompromised radiation proctitis mouse model (Guha), studying the prometastatic effects of neoadjuvant chemotherapy in breast cancer (Karagiannis), investigating the effect of tumor environment on metastasis (Oktay), the analysis of molecular markers of risk of subsequent invasive breast cancer in women with ductal carcinoma in situ (Rohan), and the study of hepatocyte heterogeneity with respect to gene transcription signatures and their zonal distribution within the intact liver in healthy tissue as well as diseased patient tissue (Singer).There are also additional projects of Minor Users and many users in the Einstein community are anticipated. All of the projects in this application are in need of high-capacity and high- reproducibility scanning so that analyses may be applied to larger tissues, covering hundreds of fields of view, rather than the single fields of view acquired on a standard microscope.