Multi-parameter, analytic flow cytometer

我们请求资金从Becton Dickinson(BD)购买一台新的5激光、30色FAC交响乐A5分析 流式细胞仪。新的A5仪器将取代我们已有15年历史的5激光18色BD LSR II仪器， 在2022年9月之后，BD将不再以服务合同提供支持。新的A5仪器将支持 对拥有51笔NIH补助金的14个主要用户和32个次要用户以及由 国防部、私营企业和基金会。我们将安装和配置新的交响乐A5 仪器与我们现有的SYMPHONY A5非常接近，这是流式细胞术中最常用的仪器 和单小区服务(FCSC)核心。通过配置新的A5仪器以匹配现有的A5仪器，我们的核心 人员和用户已经接受了操作新仪器及其机载软件的培训。此外， 已经有多参数抗体面板设计用于处理现有的交响乐A5的用户可以 在新的A5上不加修改地使用这些抗体面板，从而缓解了日程限制。基于 由于之前使用了现有的LSR II和SYNSPONY A5乐器，我们预计新的A5乐器将是 每周使用34个小时(可访问用户时间的85%，AUT)。新的A5仪器将由 FCSC核心，并将被纳入核心的现有财务计划。FCSC核心是 UAB范围的机构研究核心计划(IRCP)，因此，FSCS核心收到了重要的 来自IRCP的机构支持。三名经验丰富的流式细胞仪专家，每个人都接受过操作培训 交响乐A5可用于培训新用户操作乐器，并帮助他们 多参数抗体板的研制。最后，FCSC核心位于谢尔比生物医学中心 研究大楼，是我们大多数主要用户的研究实验室所在的大楼，位于 校园内其他用户的步行距离很短。因此，请求的新交响乐A5乐器将填充 这是FCSC核心的一个关键空白，将使我们能够继续提供最先进的仪器和服务 给我们的用户。

We request funds to purchase from Becton Dickinson (BD) a new 5-laser, 30-color FACSymphony A5 analytic flow cytometer. The new A5 instrument will replace our 15-year-old, 5-laser, 18-color BD LSR II instrument that BD will no longer support with a service contract after September 2022. The new A5 instrument will support the research of 14 major users and 32 minor users holding 51 NIH grants, as well as projects supported by the Department of Defense, private industry, and foundations. We will install and configure the new Symphony A5 instrument to closely match our existing Symphony A5, which is the most-used instrument in the Flow Cytometry and Single Cell services (FCSC) Core. By configuring the new A5 instrument to match the existing one, our Core personnel and users will be already trained to operate the new instrument and its onboard software. Moreover, users who already have multi-parameter antibody panels designed to work on the existing Symphony A5 can use these antibody panels on the new A5 without modification, thereby easing scheduling constraints. Based on the prior use of the existing LSR II and Symphony A5 instruments, we project that the new A5 instrument will be used 34 hours/week (85% of the Accessible User Time, AUT). The new A5 instrument will be administered by the FCSC Core and will be incorporated into the Core's existing financial plan. The FCSC Core is part of the UAB-wide Institutional Research Core Program (IRCP) and as such, the FSCS Core receives significant institutional support from the IRCP. Three highly experienced flow cytometry specialists, each trained to operate the Symphony A5, are available to train new users in instrument operation and to assist them with the development of multi-parameter antibody panels. Finally, the FCSC Core is located in the Shelby Biomedical Research Building, a building that houses the research laboratories of most of our major users and is within a short walking distance of our other users on campus. Thus, the requested new Symphony A5 instrument will fill a critical void in the FCSC Core and will allow us to continue to provide state-of-the-art instruments and services to our users.