RReSTORE: RGC Repopulation, Stem Cell Transplantation, and Optic Nerve Regeneration.

项目总结 视神经再生具有恢复数百万青光眼患者视力的潜力 其他视神经疾病，目前是世界上导致不可逆转失明的主要原因。取代研资局 提出了一个巨大的挑战，因为RGC代表了一类不同种类的投射神经元 在精确编码视觉刺激的区隔特征的同时进行远距离编码。视力恢复中的 视神经病变需要恢复视网膜内和多个视网膜丘脑的RGC连接 目标。虽然在实现部分再生方面取得了重大进展，但RGC途径在 分离，将这些发现转化为针对整个RGC途径的可行临床疗法 需要协作的多学科团队来讨论方法、共享数据和统一协议。 我们建议通过召集一个由大约150名具有不同背景的调查人员组成的小组来启动这一进程 解决视神经再生中最紧迫的问题的背景和科学专业知识。 视网膜神经节细胞再生、干细胞移植与视神经再生 (RReSTORe)研讨会将召集约150名领先的和新兴的视觉科学家1)定义并确定优先顺序 与视神经疾病再生医学相关的最关键的挑战和问题 未来5年和2)集思广益创新工具和试验性方法来应对这些挑战 同时促进不同调查人员之间的合作科学调查机会。协作性 发展将分三个阶段进行。参与者将自行选择5个讨论部分，每个讨论部分由共同主持 由资深和初级研究人员组成的团队：1)RGC的发展和分化；2)移植方法和 模型；3)RGC存活和宿主相互作用；4)视网膜内部连接；5)大脑连接。在第一阶段， 参与者将描述阻碍视力临床翻译的最重要的问题和挑战 视神经病变的修复治疗，通过参与一个为期4个月的虚拟讨论，包括 迭代、包容、建立共识。在第二阶段，参与者将在RReSTORe研讨会上会面，分享 协作圆桌讨论中的想法、见解和数据，以确定工具、模型和实验 这将推动视力恢复视神经疾病治疗的临床翻译。在第三阶段，参与者将 建立一个可持续的、协作的调查员网络，以促进研资局更替领域的发展， 强调最大限度地增加参与者的多样性。基于网络的协作平台，促进讨论 和汇报，将在RReSTORe研讨会之后进行。用于对话的讨论板和季刊 将建立网络会议，并无限期地积极主持。我们的目标是为 由来自不同背景和所有人的大型、可持续的、跨学科的调查人员组成的联盟 职业生涯阶段(特别是初级科学家)为实现目标而进行沟通和协作 可持续地超越RReSTORe车间的RGC更换和再生。

PROJECT SUMMARY Optic nerve regeneration holds potential for restoring vision for millions of patients with glaucoma and other optic neuropathies, currently the world's leading cause of irreversible blindness. RGC replacement poses a formidable challenge because RGCs represent a heterogeneous class of projection neuron spanning great distances while encoding precisely compartmentalized features of visual stimuli. Vision restoration in optic neuropathy necessitates restoring RGC connectivity both within the retina and at multiple retinothalamic targets. While major advancements have been made in attaining regeneration of portions the RGC pathway in isolation, translating these discoveries to viable clinical therapies that target the entire RGC pathway requires collaborative multidisciplinary teams to discuss approaches, share data, and unify protocols. We propose to jumpstart this process by bringing together a group of ~150 investigators with diverse backgrounds and scientific expertise to solve the most pressing questions in optic nerve regeneration. The Retinal ganglion cell Repopulation, Stem cell Transplantation, and Optic Nerve Regeneration (RReSTORe) Workshop will assemble ~150 leading and emerging vision scientists to 1) define and prioritize the most critical challenges and questions related to regenerative medicine for optic nerve disease over the next 5 years and 2) brainstorm innovative tools and experimental approaches to meeting these challenges while fostering opportunities for collaborative scientific investigation among diverse investigators. Collaborative development will occur in three phases. Participants will self-select 5 discussion sections, each co-moderated by a team of senior & junior investigators: 1) RGC development & differentiation; 2) Transplantation methods & models; 3) RGC survival & host interactions; 4) Inner retinal wiring; and 5) Brain connectivity. In Phase I, participants will delineate the most important questions and challenges hindering clinical translation of vision restoration treatments for optic neuropathy, by engaging in a 4-month long virtual discussion that involves iterative, inclusive, consensus building. In Phase II, participants will meet at the RReSTORe workshop to share ideas, insights, and data in a collaborative roundtable discussion to identify tools, models, and experiments that will propel clinical translation of vision restoring optic neuropathy treatments. In Phase III, participants will establish a sustainable, collaborative investigator network that will advance the RGC replacement field, with an emphasis on maximizing diversity of participants. A web-based collaborative platform, facilitating discussions and debriefings, will follow the RReSTORe workshop. A discussion board for conversations and a quarterly webconference will be established and actively moderated indefinitely. We aim to lay the foundation for a large, sustainable, interdisciplinary consortium of investigators from diverse backgrounds and all career stages (especially junior scientists) to communicate and collaboratively work to reach the goal of RGC replacement and regrowth, sustainably beyond the RReSTORe workshop.