Fusion protein based biopesticides for sustainable crop production

该项目的目标是从传递杀虫毒素的融合蛋白和跨越目标昆虫肠道的融合蛋白中开发新型生物杀虫剂。该项目的结果将是创造有针对性的融合蛋白杀虫剂；这需要开发经济生产它们的工艺，加快表达速度，并优化相关的发酵和冷冻干燥。具体目标是：(1)生产“第一候选”融合蛋白(2)增加表达和发酵(3)研究下游加工和冷冻干燥(4)提供最大效力和货架稳定性的递送配方(5)进行所有温室/田间试验，以确定在最终用户条件下的效力，提供提交注册的数据，包括对非目标生物体的影响(6)应用这些过程来简化后续候选融合蛋白的开发

This project aims to develop novel bio insecticides from fusion proteins that deliver insecticidal toxins and across the target insect gut. The results of the project will be the creation of targeted fusion protein insecticides; this requires the development of processes for their economic production, acceleration of expression rate, and optimisation of associated fermenting and freeze drying. The detailed objectives are: (i) production of a "first candidate" fusion protein (ii) increase in expression and fermentation (iii) investigation of downstream processing and freeze drying (iv) delivery formulation for maximum efficacy and shelf stability (v) conduct all glasshouse/field trials to establish efficacy under end user conditions providing data for submission for registration, including effects on non target organisms (vi) application of these processes to streamline the development of follow up candidate fusion proteins