Symptomless Infection of Barley:resistance breeding and integrated crop protection strategies (SIBLINGS)

尽管杀菌剂的应用价值超过2500万GB，但‘Rhynchosporium’仍然是大麦最具问题和最具经济破坏性的病害，每年导致约700万GB的产量损失。研究表明，在症状出现之前，甚至在抗病品种上，这种病原菌的广泛生长具有重要意义，这改变了我们对其流行病学的理解。此前，我们仅根据可见的症状来理解耐药性--这一新知识解释了管理疾病的一些困难。了解症状前定植对产量的影响及其对疾病管理的影响至关重要，我们的育种和作物保护战略需要改变，以利用这一新知识。我们将识别、表征和结合大麦的抗性来源，以提高耐久性，并利用不同防御机制的作用模式知识来改进作物保护策略，以提高现有杀菌剂的有效性。利用寄主植物基因标记和荧光标记的病原菌分离物的显微镜方法，我们将确定抗性来源，识别候选基因组位置，并获得侧翼诊断分子标记。这些知识将用于开发新品种和验证针对这一重要病原体的最佳疾病管理方案。

Despite fungicide applications valued at over £25M, 'Rhynchosporium' continues to be the most problematic and economically damaging disease of barley leading to annual yield losses of ~£7M. Research has shown the importance of extensive growth of this pathogen before symptoms are visible, even on resistant cultivars, and this has changed our understanding of its epidemiology. Previously we based our understanding of resistance on visible symptoms only - this new knowledge explains some of the difficulties in managing the disease. Understanding the impact of pre-symptomatic colonisation on yield and its impact on disease management is pivotal and our breeding and crop protection strategies need to change to exploit this new knowledge. We will identify, characterise and combine sources of barley resistance to improve durability and use knowledge of the mode of action of different defence mechanisms to improve crop protection strategies to increase the effectiveness of currently available fungicides. Using host plant gene markers together with microscopy methods using fluorescently-tagged pathogen isolates we will characterise sources of resistance, identify candidate genomic locations and obtain flanking diagnostic molecular markers. This knowledge will be used to develop new varieties and to validate optimal disease management programmes against this important pathogen.