Accessible label-free optical microscopy with quantitative molecular and functional contrast

项目摘要/摘要： 实验室研究概述：光学成像和光谱(OIS)实验室的重点是 发展和发展光学技术，以帮助提高对生物过程和 辨别疾病的能力。具体来说，OIS实验室专注于无标签成像、线性和非线性光谱- 复制，以及先进的信号处理技术，以获得新形式的功能和分子在- 编队适用于各种应用。到目前为止，这些努力已经导致了光谱光学合作的进展。 相干显微镜(SOCT)，定量相位成像(QPI)，非线性泵浦-探测显微镜，以及 迟拉曼散射(SRS)。该实验室已经开创了几种新的分子对比形式，包括分子对比。 球面重定向、非线性相位色散光谱、紫外高光谱成像和色散-- 基于SRS的。该实验室还开发了新的计算方法来分析光谱和形态 以无人监督的方式提供功能。最后，这些方法已被应用于帮助细胞识别和 表型鉴定、基础生物学研究、疾病检测和分期等。 未来五年的目标和研究计划的总体愿景：最近，OIS实验室率先 定量斜背照相显微镜(QOBM)，并在深紫外光方面取得了重要进展。 让(紫外线)显微镜作为一种手段来解决生物学和医学中的关键挑战。在过去五年中 这项提议，这项计划寻求探索这些极具前景的新工具的能力。通过一个 结合系统/硬件和计算/软件开发，建议的计划将显示 这些无标记光学方法在揭示关键的分子、功能和结构方面的巨大作用 目前无法获得或需要昂贵或复杂的系统的细节。主 工作的重点是技术开发，但它也将探索一些应用程序，这些应用程序在 迫切需要这种无标签、非侵入性、低成本、易于使用的成像方法。 拟议的计划将带来一个强大的平台，将指导未来的创新。一个明显的例子就是搭售 在新兴计算方法的巨大威力中，这种用于图像翻译的深度神经网络- 分类和分类。此外，随着拟议工具的出现以及对其更好的理解 功能，研究人员将能够进行更有针对性的工作，为特定应用定制这些工具- 阳离子。因此，拟议的工作和它将在未来实现的能力可能具有巨大的潜力 改善和影响生物和医学。

Project Summary/Abstract: Overview of research in the laboratory: The Optical Imaging and Spectroscopy (OIS) laboratory focuses in de- veloping and advancing optical technologies to help improve the understanding of biological processes and the ability to identify disease. Specifically, the OIS lab focuses on label-free imaging, linear and nonlinear spectros- copy, and advanced signal processing techniques to gain access to novel forms of functional and molecular in- formation for a variety of applications. To date, these efforts have led to advances in spectroscopic optical co- herence microscopy (SOCT), quantitative phase imaging (QPI), nonlinear pump-probe microscopy, and stimu- lated Raman scattering (SRS). The lab has pioneered several new forms of molecular contrast, including molec- ular reorientation, nonlinear phase dispersion spectroscopy, ultraviolet hyperspectral imaging, and dispersion- based SRS. The lab has also developed new computational methods for analyzing spectral and morphological features in an unsupervised manner. Finally, these methods have been applied to help in cell identification and phenotyping, basic biological studies, disease detection and staging, and more. Goals for the next five years, and the overall vision of the research program: Recently, the OIS lab pioneered quantitative oblique back illumination microscopy (qOBM) and has made important advances in deep ultravio- let (UV) microscopy as a means to address critical challenges in biology and medicine. Over the five years of this proposal, this program seeks to explore the capabilities of these highly promising new tools. Through a combination of system/hardware and computational/software developments, the proposed program will show the tremendous utility of these label-free optical methods to reveal critical molecular, functional, and structural detail that is currently impossible to obtain or requires prohibitive expensive or complex systems.. The main focus of the work is on technology development, but it will also explore a number of applications that are in dire need of such label-free, non-invasive, low-cost, easy-to-use imaging methods. The proposed program will lead to a robust platform that will guide future innovation. A clear example is tying in the tremendous power of emerging computational methods, such deep neural networks for image transla- tion and classification. Additionally, with the advent of the proposed tools and a better understanding of their capabilities, researchers will be able to venture into more targeted effort to tailor these tools for specific appli- cations. Thus, the proposed work and the capabilities it will enable in the future can have tremendous potential to improve and influence biology and medicine.