Development of in-farm diagnostic tests for Campylobacter

背景这项研究工作是由一个由研究和工业技术专家、科学家和家禽生产者组成的联盟进行的。目的是开发一种快速检测系统，可以非常准确地用于在农场评估鸡群是否在其内脏道中携带空肠弯曲杆菌或大肠弯曲杆菌。该细菌不会伤害鸟类，但却是一种主要的人类病原体，在英国每年造成70.000至300.000例病例的爆发。我们相信，通过更多的知识，通过屠宰厂有针对性的响应，增加屠体清洗和其他工厂干预措施的发展，我们可以将人类感染率降低高达70%，为卫生服务部门节省大量资金，并减少因停工而造成的人力成本。迄今为止取得的进展已经进行了选择性研究，以证明灯检测过程可以显示是否存在大肠杆菌或空肠弯曲菌。进一步的测试表明，分析仪对C Coli的反应与对S Aureus，L Monocytogenes和S Epidermis的反应有很大的不同。所有这些测试都取得了成功。然后，我们开始评估弯曲杆菌细胞的捕获水平，我们仍然可以获得独特的阳性测试结果，将微生物收集在包被有靶细菌抗原的珠子上。退火温度下的结果。进一步的工作已经进行，以显示测试对使用包被珠子选择的样品的影响，然后加热样品以杀死并分解目标生物体的DNA。这个测试组也是有效的，目标生物体的一个独特而清晰的图，并没有从其他细菌测试弯曲杆菌的效果。进展我们很高兴在第一季度的工作进展和成功，并取得了比我们预期的更好的进展。我们已直接从火鸡农场收集粪便样本，并正进行进一步测试。第二季的目标。我们会进行额外的验证工作，以核实检测方法的选择性，并确定检测方法的检出限。一个内部的扩增控制也将被构建并整合到检测中。我们希望完成仪器的设计规范，并继续进行电子学的开发。这个概念开始看起来像一个农场友好的过程，可以提供准确的结果，即使是从轻度感染的鸡群，具有非常好的准确性，并在一个小时内完成测试。杰里米霍尔-首席协调员和行业领导。2012年7月13日

BackgroundThe research work is being undertaken by a consortium of research and industry based technologists, scientists and poultry producers. The aim is to develop a rapid test system that can be used with great accuracy to evaluate on the farm whether the flock is carrying in its visceral tract the campylobacter’s of C Jejuni or C Coli.The bacterium does not harm the birds but is a major human pathogen causing outbreaks at the level of between 70.000 and 300.000 cases per year in the United Kingdom.We believe that via greater knowledge and a targeted response in the slaughter plant with increased carcass washing and the development of other factory interventions, we can reduce the human infection rate by up to 70%, saving the Health service considerable sums and reducing the human cost of many lost days off work.Progress to date.Selective studies have been undertaken to prove that the lamp assay process can show when either C Coli or C Jejuni are present. Further tests have then been undertaken to show that the response on the analyser to C Coli is dramatically different to that for S Aureus, L Monocytogenes, and S Epidermis. All of these tests have been successful.We have then moved to assess at what levels of capture of the Campylobacter cells can we still get a distinctive and positive test result, having collected the organisms on beads encrusted with the antigen for the target bacteria.Results at annealing temperatures.Further work has been undertaken to show the effects of the tests on samples that have been selected using coated beads, and then the samples are heated to kill and break down the DNA of the target organism. This test group was also effective with a distinct and clear plot for the target organism and with no effect being seen from other bacteria tested alongside the campylobacter’s.ProgressWe are pleased with the progress and success of the work in the first quarter, and have progressed better than we had expected. Samples of faecal samples have been collected direct from turkey farms and further tests are being undertaken.Second Quarter targets.Additional verification work will be undertaken to verify the selectivity and to determine the detection limit of the assay. An internal amplification control will also be constructed and incorporated into the assay.We hope to complete the instrument design specification, and continue with the electronics development. The concept is starting to look like a farm friendly process that can deliver accurate results even from lightly infected flocks, with very good accuracy, and with tests completed in well under an hour.JEREMY HALL - Lead Co-ordinator and Industrial lead.13 July 2012