GENETIC AND MOLECULAR BASIS OF BACTERIAL INVASION

我们提出的研究的目的是为了更好地了解 细菌-宿主细胞相互作用的遗传和分子基础。我们 重点关注这种联系的三个方面：细菌的进入 (耶尔森氏菌和沙门氏菌)进入培养的上皮细胞和巨噬细胞； 随后对细胞间和跨细胞通路的分析 这些入侵细菌在明确的细胞培养模型中；以及 这些细菌在M细胞和Peyer‘s斑块中的相互作用 敏感和抵抗力强的动物。我们将继续调查 致病性的遗传和分子决定因素允许这些 微生物进入细胞或防止它们被细胞和 幽默主持人因素。我们在这些领域的前期工作已经是最多的 令人鼓舞，并表明微生物致病的这些方面是 服从卓有成效的实验研究。

The object of our proposed research is to gain a better understanding of the genetic and molecular basis of bacterial-host cell interactions. We focus on three aspects of this association: the entry of bacteria (Yersinia and Salmonella) into cultured epithelial cells and macrophages; the analysis of the intercellular and transcytotic pathways followed by these invasive bacteria in well-defined cell culture models; and the interactions of these bacteria in the M cells and Peyer's patches of susceptible and resistant animals. We continue to investigate the genetic and molecular determinants of pathogenicity that permit these microbes to enter cells or to prevent their destruction by cellular and humoral host factors. Our preliminary work in these areas has been most encouraging and suggests that these aspects of microbial pathogenesis are amenable to fruitful experimental investigation.