PEPTIDE GROWTH FACTORS AND SKELETAL DEVELOPMENT

描述：（改编自研究者摘要）几乎所有 骨骼的生长是细胞有序分裂和成熟的结果。 骨骺生长板的细胞 正常骨骼发育 取决于对这个过程的正确控制。 这项建议是 基于申请人先前的观察， 这些因素参与了控制。 拟议研究的目标 是阐明选择的生长因子调节 生长板软骨细胞 研究人员假设， 因子互作在生长调节中起重要作用 板状软骨细胞 这些研究的目的之一就是检验这一假设 通过研究选择性增长的个体效应和交互效应， 与有丝分裂和分化的软骨细胞功能有关的因素 体外 细胞培养，代谢标记，放射免疫测定， 将使用免疫抑制、北方和原位杂交方法 以确定相互作用，并开始阐明其机制。 的 第二个目的是检验这一假设，即促钙剂的作用 生长板软骨细胞上的激素，降钙素和PTH， 肽生长因子。 具体而言，调节软骨细胞PTH 受体和降钙素受体表达的选择生长因子 将通过细胞生物学和分子生物学技术进行检测。 第三个目的是评估外源性管理的能力， 生长因子调节体内骨骼生长。使用大鼠模型， 选定因素的作用和相互作用将通过 代谢和原位杂交技术。 长期目标 这些研究的目的是帮助人们对 调节骨骼生长的机制。只有掌握了这些信息， 骨骼生长和修复的异常， 生长因子最终对临床问题的潜在贡献 被确定。

DESCRIPTION: (Adapted From Investigator's Abstract) Virtually all skeletal growth is the result of the orderly division and maturation of the cells of the epiphyseal growth plate. Normal skeletal development is dependent on the proper control of this process. This proposal is based on the applicants' previous observations that peptide growth factors participate in this control. The goal of the proposed studies is to elucidate the mechanisms by which selected growth factors regulate growth plate chondrocytes. The investigators hypothesize that growth factor interaction plays an important role in the regulation of growth plate chondrocytes. One aim of these studies is to test this hypothesis by examining the individual and interactive effects of selected growth factors with respect to mitotic and differentiated chondrocyte functions in vitro. Cell culture, metabolic labelling, radioimmunoassay, immunoinhibition, Northern and in situ hybridization methods will be used to identify interactions and begin to elucidate their mechanisms. The second aim is to test the postulate that the effects of the calciotropic hormones, calcitonin and PTH, on growth plate chondrocytes are modulated by peptide growth factors. Specifically, regulation of chondrocyte PTH receptor and calcitonin receptor expression by selected growth factors will be examined by cell biological and molecular biological techniques. The third aim is to assess the ability of exogenously administered growth factors to regulate skeletal growth in vivo.Using a rat model, the action and interaction of selected factors will be evaluated by metabolic and in situ hybridization techniques. The long-term objective of these studies is to help achieve a basic understanding of the mechanisms regulating skeletal growth. Only with such information can abnormalities of skeletal growth and repair be understood and the potential contribution of growth factors to clinical problems eventually be determined.