FACTORS INFLUENCING SURVIVAL OF VASCULARIZED BONE GRAFTS

本申请的具体目的是继续调查 影响血管化骨移植原则的因素。 目前可用的血管化骨移植物用于重建巨大的 广泛切除后受损骨床的节段性骨丢失 恶性骨肿瘤、创伤性骨缺损和感染性骨不连 由于结构确认， 移植物和供区发病率。 这项提议的中心目标是 是利用生物合成成分（羟基磷灰石陶瓷模具）， 骨诱导成分和骨祖细胞与 血管蒂，以产生“一个成型的血管化骨生成”。 本提案的第二个目标是分离骨髓基质细胞， 骨祖细胞，确定适当的程度， 分化，最大限度地提高体内成骨，并扩大这一点， 在用于模制成骨之前，离体确定的群体。 研究旨在分离表征骨祖细胞 细胞，并利用扩散室， 确定与基质宿主细胞接触的适当水平， 确定脱钙骨基质和生长因子的作用 异位性骨再生 这种血管化的骨移植物 将被用作岛状移植或自由转移， 将检查这些移植物的材料特性，并与 传统的骨移植。

The specific aims of this application are to continue the investigation of factors influencing the principles of vascularized bone grafts. Currently available vascularized bone grafts used to reconstruct massive segmental bone loss in compromised beds following wide resections of malignant bone tumors, traumatic bony defects, and infected non-unions have significant limitations resulting from structural confirmation of the graft, and donor site morbidity. The central goal of this proposal is to utilize biosynthetic components (hydroxyapatite ceramic mold), osteoinductive principles and osteoprogenitor cells coupled with a vascularized pedicle to produce "a molded vascularized osteogenesis". The second goal of this proposal is to isolate bone marrow stromal osteoprogenitor cells, determine the appropriate degree of differentiation that maximizes osteogenesis in vivo and expand this to defined populations ex vivo prior to utilization in molded osteogenesis. Studies have been designed to isolate the characterize osteoprogenitor cells from rabbit marrow in vitro and utilize diffusion chambers to determine the appropriate level of contact with matrix host cells and to determine the effects of demineralized bone matrix and of growth factors on heterotopic bone regeneration. This vascularized molded bone graft will be used as an island graft or a free transfer and the healing and material properties of these grafts will be examined and compared to conventional bone grafts.