BUNYAVIRUS/MOSQUITO INTERACTIONS

加州的本雅群病毒有其特殊性。 蚊子。因为这种载体的特异性，即使当两个人 脑炎病毒如La Crosse和詹姆斯敦峡谷存在于 在同一地区，它们维持着不同的生命周期。最近推出的 非本地蚊子白纹伊蚊可以感染这两种病毒。 此外，这种蚊子正在美国的许多地区定居，在那里 这两种病毒都存在。似乎有理由认为这种蚊子 会同时感染这两种病毒，这种混合感染会导致 JC/LACV重组病毒的产生。中国的长期目标是 拟议的研究是为了了解本雅氏病毒与其 蚊子媒介。具体地说，研究的目的是：1)确定 LACV和JCV在昆虫传播上差异的遗传基础 利用重组病毒2)确定基因组的需求 哺乳动物感染、扩增和神经病理学中的片段3)至 确定LACV G2上负责病毒结合的表位 蚊子细胞，并确定所涉及的氨基酸序列和 检查其他布尼亚病毒类似的表位。此外，这些 重新组合可能会对公共健康造成重大威胁，并对 这些病毒及其媒介应该使我们能够评估这种风险。未来 研究可能会利用这一知识来预防本亚病毒。 使用转基因蚊子等技术传播疾病。

There is specificity of California serogroup bunyaviruses for particular mosquitoes. Because of this vector specificity even when two human encephalitis viruses such as La Crosse and Jamestown Canyon exist in the same area, they maintain separate life cycles. The recently introduced non-native mosquito Aedes albopictus can be infected with both viruses. Moreover, this mosquito is colonizing many areas of the United States where both viruses are present. It seems reasonable to assume that this mosquito will become infected with both viruses and this co-infection will result in the generation of JC/LACV reassorted viruses. The long term goal of the proposed research is to understand the interaction of a bunyavirus with its mosquito vector. Specifically, the research aims are: 1) to determine the genetic basis for differences in insect transmission of lACV and JCV by the use of reassorted viruses 2) to determine the requirement of genome segments in infection, amplification and neuropathology in mammals 3) to identify epitopes on lACV G2 responsible for attachment of the virus to mosquito cells and determine the amino acid sequences involved and to examine other bunyaviruses for similar epitopes. Moreover, these reassortants may have a significant public health threat and examination of these viruses and their vector should allow us to assess this risk. Future studies may exploit this knowledge for the prevention of bunyavirus diseases using techniques such as transgenic mosquitoes.