TARGETED DISRUPTION OF THE MOUSE CALCITONIN GENE

尽管已知降钙素(CT)是一种药理抑制剂 骨吸收和降钙素基因相关产物(CGRP)是一种有效的 血管扩张剂和神经肽及其相关的生理作用 多肽仍未得到证实。CALC I和CALC II基因编码CGRP。 CALC I的转录导致CT和CGRP-I的mRNA编码。 Calc II只产生CGRP(CGRP-II)。这两种降钙素基因相关肽具有很高的 与独特的、有时重叠的组织分布同源。至 解剖这些密切相关的荷尔蒙对正常的贡献 哺乳动物发育和生理学，动物模型包含NULL 每个基因座都会产生突变。为了做到这一点，每个基因 将使用靶向基因技术选择性地消除 破坏多能小鼠胚胎干细胞。

Although it is known that calcitonin (CT) is a pharmacological inhibitor of bone resorption and calcitonin gene-related product (CGRP) is a potent vasodilator and neuropeptide, the physiologic role for these related peptides remains unproved. The CALC I and CALC II genes encode for CGRP. Transcription of CALC I results in an mRNA coding for both CT and CGRP-I. CALC II produces only CGRP (CGRP-II). The two CGRP peptides are highly homologous with unique, at times overlapping, tissue distributions. To dissect the contributions of these closely related hormones to normal mammalian development and physiology, animal models containing null mutations at each locus will be generated. To accomplish this, each gene will be selectively eliminated using the technique of targeted gene disruption in pluripotent mouse embryonic stem (ES) cells.