NEUROPHYSIOLOGY OF THE LUMBAR DISC AND ADJACENT TISSUE

这个项目的长期目标是了解神经生理学。 包括药物的相对有效性的下腰痛 治疗方式。初步研究将集中在外围设备上 椎管结构的神经支配和神经生理学。后来 研究将包括更高级的神经元，并在背部进行记录 角、丘脑和躯体感觉皮质。椎管结构至 被研究 包括椎间盘、后纵韧带、硬脑膜腹侧、后根 和背根神经节。长期以来，人们一直怀疑这些组织 腰痛的来源，但他们的神经从来没有 具有神经生理学特征的。这项建议的目的是 确定这些组织的体感神经元如何对 机械应力和应变以及疼痛的化学介质和 发炎。我们将使用的模型是新英格兰白兔。 具体目标： 1.测定腰椎间盘的神经元反应， 后纵韧带、硬脑膜腹侧、背根和背侧 根神经节受局部机械应力的影响。 2.测定间盘的神经元反应 正在装车。 3.确定这些组织对已证实的和 假想的炎症化学介质，包括突出物 髓核，磷脂酶A2，P物质，缓激肽和 血清素。 4.测定体感神经肽在脑内的神经分布 这些纸巾。 假设： 1.腰痛可能是由机械或化学刺激引起的 在椎间盘环内的伤害性感受器，后纵向 韧带或腹侧硬脑膜。这一假设从未被证实过。 神经生理学上。 2.腰背痛和坐骨神经痛可由机械压力或 腰背根和/或背根神经节的炎症。

The long term goals of this project are to understand the neurophysiology of low back pain including the relative effectiveness of pharmacologic treatment modalities. The initial studies will focus on peripheral innervation and neurophysiology of spinal canal structures. Later studies will include higher order neurons, with recordings made at dorsal horn, thalamus and somatosensory cortex. The spinal canal structures to be studied include disc, posterior longitudinal ligament, ventral dura, dorsal roots and dorsal root ganglia. These tissues have long been suspected to be sources of low back pain, but their innervation has never been characterized neurophysiologically. The purpose of this proposal is to determine how the somatosensory neurons of these tissues respond to mechanical stress and strain and chemical mediators of pain and inflammation. The model we will use is the New England White rabbit. Specific Aims: 1. Determine the neuronal response of lumbar intervertebral discs, posterior longitudinal ligaments, ventral dura, dorsal roots and dorsal root ganglia to local mechanical stress. 2. Determine the neuronal response of the intervertebral disc to disc loading. 3. Determine the response of these same tissues to exposure to proven and hypothesized chemical mediators of inflammation, including herniated nucleus pulposus, phospholipase A2, substance P, bradykinin and serotonin. 4. Determine the nerve distribution of somatosensory neuropeptides in these tissues. Hypotheses: 1. That low back pain can be caused by mechanical or chemical stimulation of nociceptors in the annulus of the discs, posterior longitudinal ligament or ventral dura. This hypothesis has never been demonstrated neurophysiologically. 2. That low back pain and sciatica can be caused by mechanical stress or inflammation of lumbar dorsal roots and/or dorsal root ganglia.