Investigating the biogenesis and function of circular RNAs in the brain

环状RNA （circRNAs）是广泛存在于真核生物（从酵母到人类）中的一类新型RNA分子。它们在果蝇、老鼠和人类的神经系统中的表达尤其高。环状RNA表达的组织特异性富集机制是什么？环状RNA的产生对基因表达的影响是什么？拟议项目的总体目标是阐明circRNA生物发生的这些重要方面。具体来说，该项目旨在(a)发现调节circRNA表达的蛋白质，(b)阐明circRNA表达如何与选择性剪接相互作用，以及(c)鉴定在基因表达中发挥调节作用的环状rna。

Circular RNAs (circRNAs) are e a novel class of RNA molecules produced in a wide spectrum of eukaryotic organisms, from yeast to humans. Their expression is particularly high in the nervous system in the fruit fly, mouse and humans. What mechanisms are responsible for the tissue-specific enrichment of circular RNA expression? What are the consequences of circular RNA production on gene expression? The overall goal of the proposed project is to elucidate these important aspects of circRNA biogenesis. Specifically, the project aims to (a) discover proteins that regulate circRNA expression, (b) elucidate how circRNA expression interacts with alternative splicing, and (c) identify circular RNAs that play regulatory roles in gene expression.