AUTOMATED THERAPY MONITORING IN LEUKEMIA CYTOGENETICS

该项目的目标是开发一套显著改进的 细胞遗传学标本制备技术和自动图像分析 用于诊断白血病和监测进展的硬件和软件， 对治疗的反应和疾病的缓解。 这些技术会奏效的 在间期细胞上，避免了培养中期细胞的需要。 这 将减少白血病诊断的时间和成本要求， 治疗监测，并提供更多的定量数据用于诊断， 治疗 具体来说，我们将制定骨髓标本制备、染色 标签技术和自动图像分析硬件和软件 基于以下因素对间期细胞的比例进行破碎和定量 (10细胞遗传学正常与异常，（2）增殖指数和（3） 细胞成熟状态。 我们将使用（1）间期核上的DNA探针来确定哪些细胞 有特定的白血病相关的染色体异常，（2）抗BrdU 与荧光团缀合以确定增殖指数，和（3） 计算机形态图像分析评价细胞状态 成熟 分析将在自动显微镜上进行， 过程60在无人值守的操作中滑动。 这种新的能力将大大提高临床医生的能力， 来诊断白血病和监测治疗。

The goal of this project is to develop a set of significantly improved cytogenetics specimen preparation techniques and automated image analysis hardware and software for diagnosing leukemia and monitoring the progress, response to therapy and remission of the disease. The techniques will work on interphase cells, avoiding the need for culturing metaphase cells. This will reduce the time and cost requirements of leukemia diagnosis and therapy monitoring and provide more quantitative data for diagnosis and treatment. Specifically, we will develop bone marrow specimen preparation, staining and labeling techniques and automated image analysis hardware and software to fractionate and quantify the proportions of interphase cells based on (10 cytogenetically normal versus abnormal, (2) proliferation index and (3) state of cell maturity. We will use (1) DNA probes on interphase nuclei to determine which cells have specific leukemia-related chromosomal abnormalities, (2) Anti-BrdU conjugated with a fluorophore to determine proliferation index, and (3) computerized morphological image analysis to evaluate state of cell maturity. The analysis will be done on an automated microscope that can process 60 slides in unattended operation. This new capability will significantly increase the ability of clinicians to diagnose leukemia and monitor therapy.