CellulaREvolution: Bringing Continuous Processing to Cell Manufacture

“有一些成熟的和新兴的商业市场利用培养活细胞的科学进步来‘制造’新产品，包括药物、干细胞、基因疗法和基于细胞的肉类。致力于这些开发的公司正在应对未来几十年世界面临的一些主要挑战，如全球饥饿、全球变暖和流行病威胁。其中许多应用依赖于贴壁细胞的使用(细胞需要固体表面才能生长，如培养瓶底部)。培养贴壁细胞的标准方法50年来没有改变；细胞是分批培养的，每批细胞的大小受到细胞附着的表面积的限制(例如。烧瓶大小)。关键是，用于贴壁细胞培养的传统批量生物处理技术不能满足当前的需求(典型批次大小为10e^10个细胞，但在一些市场的需求更多在10e^30)，因此是生产或使用贴壁细胞的公司未来商业潜力的重要瓶颈。一种替代通过使用微载体、中空纤维或多层烧瓶来简单地增加表面积的方法，因为这些方法实际上永远不能满足不断增长的需求。在CellulaREvolution，我们的目标是建立一种革命性的方法，在无血清培养基中培养贴壁细胞。我们的新方法是从传统的分批培养(其过于复杂的增加表面积的方法)转变为简单的连续培养系统，其中细胞不断地产生。我们的创新首次为这种方法提供了便利。有鉴于此，目前的提案将使CellulaRvolution能够通过任命首席执行官、聘用合格的团队成员、租用实验室/办公室、支付咨询、法律费用和进一步的市场研究来建立业务。在这个阶段加快公司发展，将有助于CellulaREvolution吸引私人投资者为其产品的初步开发和销售提供资金支持。“

"There are a number of well-established and emerging commercial markets exploiting scientific advances in growing living cells to 'manufacture' new products, including pharmaceutical drugs, stem cells, gene therapies and cell-based meat. Companies working on these developments are addressing some of the major challenges facing the world over the coming decades, such as global hunger, global warming and pandemic threats.Many of these applications rely on the use of adherent cells (cells requiring a solid surface on which to grow, such as the bottom of a culture flask). The standard approach to growing adherent cells has not changed in 50 years; cells are grown in batches, the size of each batch limited by the surface area upon which the cells are attached (_e.g_. flask size). Critically, traditional batch bioprocessing techniques for the culture of adherent cells cannot meet current demands (typical lot sizes are 10e^10 cells but needs in some markets are more in the range of 10e^30) and is therefore a significant bottleneck to the future commercial potential of companies that produce or use adherent cells.A radical departure from traditional engineering solutions is required, _i.e_. an alternative to simply increasing surface area by using microcarriers, hollow fibres or multi-layered flasks, as these approaches can never practically meet the growing demand. At CellulaREvolution we aim at establishing a revolutionary method to culture adherent cells in serum-free medium. Our novel approach is to move from the traditional batch culture (with its overly complicated ways to increase surface area) to a simple continuous culture system where cells are produced unremittingly. Our innovation facilitates such an approach for the first time.In light of this, the current proposal will enable the setting up of CellulaREvolution as a business via the appointment of a CEO, hiring qualified team members, renting lab/office space, paying for consultancy, legal costs and further market research. Accelerating the company development at this stage, will help CellulaREvolution to attract private investors to financially support initial development and sales of its products."