LOCALIZATION AND REGULATION OF THE RAF-1 PROTEIN KINASE

Raf-1是一种位于中央位置的丝氨酸/苏氨酸特异性蛋白激酶 关于调节细胞生长的信号转导级联反应 一系列多样化的增长因素。此外，Raf-1可以是 通过截断调节性N-末端而激活致癌 领域建议的实验将定义Raf-1N-1中的那些区域 与膜稳定结合所必需的末端。 此外，它们将更准确地描述 Raf-1的N末端是激活和调节Raf-1所必需的。最后， 利用嵌合蛋白，膜定位之间的关系 而Raf-1的激活将被更详细地探讨。成功者 该项目的完成将使我们更好地了解RAF-1 信号传递和有丝分裂级联的调节。新见解 由这些研究产生的Raf-1相互作用可能 可能被用作治疗的靶点，旨在破坏 需要Raf-1激活的信号，即来自致癌RAS和 激活酪氨酸激酶受体。

Raf-1 is a serine/threonine specific protein kinase centrally positioned on a signal transduction cascade regulating cell growth in response to a diverse array of growth factors. Additionally, Raf-1 can be oncogenically activated by truncation of the regulatory N-terminal domain proposed experiments will define those regions in the Raf-1 N- terminus necessary for stable association with the membrane. Furthermore, they will delineate more precisely the sequences in the Raf-1 N-terminus necessary for Raf-1 activation and regulation. Lastly, using chimeric proteins, the relationship between membrane localization and Raf-1 activation will be explored in further detail. The successful completion of this project will provide a greater understanding of Raf-1 signalling and the modulation of the mitogenic cascade. New insights into Raf-1 interactions resulting from these investigations could potentially be used as targets for therapeutics designed to disrupt signals requiring Raf-1 activation i.e., those from oncogenic Ras and activated tyrosine kinase receptors.