NEUTRAL GLYCOLIPID ANTIGENS

格林-巴利综合征(GBS)是一种免疫介导的炎症性、 脱髓鞘多发性神经病。GBS患者血清补体 可以找到针对周围神经髓鞘的固定抗体，该抗体 与临床病程相关并先于临床出现 激活脑脊液、血清和周围神经中的补体成分。一个 在一些GBS患者中，这种抗体活性的很大一部分是定向的 对抗周围神经髓鞘的中性糖脂 与福斯曼抗原发生交叉反应。Forssman是几个组件中的一个 病毒和细菌，以及伴随着许多 感染部分是针对福斯曼抗原的。在这 建议我们研究抗体和补体在 实验性变态反应性神经炎周围神经脱髓鞘 GBS的动物模型。我们的具体目标是：1)研究 Lewis大鼠实验性变态反应性神经炎的发展， 福斯曼抗原免疫后。2)确定 Forssman诱导的EAN血清抗体识别Forssman表位的研究 动物。3)确定从EAN血清中亲和纯化的抗体是否能 神经内注射致Lewis大鼠坐骨神经脱髓鞘 很有胆量。4)研究补体耗竭对血-神经的影响 周围神经髓鞘诱导的EAN屏障完整性。 将进行电生理测试和组织学研究，以评估 周围神经脱髓鞘在EAN和神经内注射后。 将使用的免疫学技术包括酶联免疫吸附试验、c1固定-转移 测定和高效薄层扫描法。

Guillain-Barre' syndrome (GBS) is an immune-mediated, inflammatory, demyelinating polyneuropathy. In serum of GBS patients, complement fixing antibodies against peripheral nerve myelin can be found, which correlate with the clinical course and precede the appearance of activated complement components in CSF, serum and peripheral nerve. A significant portion of this Ab activity in some GBS patients is directed against a neutral glycolipid of peripheral nerve myelin that cross-reacts with Forssman antigen. Forssman is a component of several viruses and bacteria, and heterophile responses accompanying many infections are partly directed against Forssman antigen. In this proposal we want to study the role of antibody and complement in peripheral nerve demyelination in experimental allergic neuritis (EAN), an animal model of GBS. Our specific aims are: 1) To study the development of experimental allergic neuritis in the Lewis rat, following immunization with Forssman antigen. 2) To determine which epitope on Forssman is recognized by serum Ab from Forssman induced EAN animals. 3) To determine if affinity-purified Ab from EAN serum can cause demyelination following intraneural injection in Lewis rat sciatic nerve. 4) To study the effect of complement depletion on blood-nerve barrier integrity in EAN induced by peripheral nerve myelin. Electrophysiologic testing and histologic studies will be done to assess peripheral nerve demyelination in EAN, and after intraneural injection. Immunological techniques to be used include ELISA, C1 fixation-transfer assay and HPTLC.