LIN-13 AND SPECIFICATION OF CELL FAT

lin-13在筛选C. elegans 外阴谱系 它的功能是限制六个等位中的三个 外阴前体细胞经历外阴细胞命运。 遗传分析 表明现有的等位基因只是部分降低了基因功能， 功能完全丧失（无效）表型可能是致死性的。 所以， lin-13还可能在发展中发挥重要作用。 我打算学习 lin-13在发育中的作用， 分子分析 为了确定lin-13在早期 在发育过程中，将分离和表征无效等位基因。 终端 将检查这些等位基因的表型，并且任何细胞谱系 将描述变换。 将进行遗传镶嵌分析， 确定细胞谱系中lin-13活性对于生存力是必需的 和外阴发育的作用 与lin-13相互作用的基因将 其特征在于两种方式：用lin-13构建双突变体 和其他已知在外阴通路中起作用的基因; 和抑制突变的表征。 最后，开始 为了研究lin-13作用的分子机制， 克隆和序列测定

lin-13 has been identified in screens for mutants defective in C. elegans vulval lineages. It functions to restrict three of the six equipotential vulval precursor cells from undergoing vulval cell fates. Genetic analysis suggests that existing alleles only partially reduce gene function, and that the complete loss of function (null) phenotype may be lethality. So, lin-13 may also have an essential role in development. I propose to study the roles of lin-13 in development using a combination of genetic and molecular analysis. To identify the role lin-13 plays early in development, null alleles will be isolated and characterized. The terminal phenotype of these alleles will be examined, and any cell lineage transformations will be described. Genetic mosaic analysis will be done to determine where in the cell lineage lin-13 activity is needed for viability and for vulval development. Genes that interact with lin-13 will be characterized in two ways: the construction of double mutants with lin-13 and other genes known to function in the vulval pathways; and the isolation and characterization of suppressor mutations. Finally, to begin investigating the molecular mechanisms of lin-13 action, the gene will be cloned and sequenced.