BEHAVIOR OF P FACTORS--MOVABLE ELEMENTS IN DROSOPHILA

长期目标：可转座的基因成分无处不在 在真核基因组中。我们的目标是 要了解这些元素如何能够插入到新的 基因组位置，从旧的位置上切除，并调节这些 活动。 具体目标： 转座：一些可转座的元件通过复制进行转座 他们自己处于新的位置，而其他人则“保守地”跳跃， 在这个过程中，捐赠者的网站被删除了。建议 实验将确定P的运动机制 果蝇中的转座子家族。 调控：已知一些P元素可以产生基因产物 这就控制了他们自己的流动性。实验将决定 这一规定的遗传要求及其与 元素的基因表达。 与健康相关：可转座基因插入被认为是 是自发突变的主要来源。这些实验 将提供有关P元素如何发挥作用的基本数据，因此 有助于了解有害物质的发生率和控制 突变。此外，P元素还被广泛用于遗传 研究基因功能一般问题的载体。结果 建议的实验可以用来增强这些 技巧。最后，人们认为昆虫是可以转座的 元素最终将被证明在对抗害虫方面是有用的。 对它们的基本生物学的了解，从 这些实验对于任何这样的努力都是必不可少的。 方法：标准遗传学与分子生物学相结合 将采用各种技术。果蝇将在 实验室和表型筛选的遗传变异 利息。分子技术包括DNA测序和 聚合酶链式反应扩增特异性DNA Se-DNA

Long-Term Objectives: Transposable genetic elements are ubiquitous in eukaryotic genomes. The goal is to understand how such elements are able to insert into new genomic positions, excise from old ones, and regulate these activities. Specific Aims: Transposition: Some transposable elements transpose by replicating themselves in new positions, whereas others jump "conservatively", with the donor site being deleted in the process. Proposed experiments will determine the mechanism of mobility for the P family of transposable elements in Drosophila melanogaster. Regulation: Some P elements are known to produce a gene product that regulates their own mobility. Experiments will determine the genetic requirements for this regulation and its relationship to the element's gene expression. Health Relatedness: Transposable element insertions are thought to be a major source of spontaneous mutations. These experiments will provide the basic data on how P elements function, and thus contribute to the understanding of the rate and control of harmful mutations. In addition, P elements are widely used as genetic vectors for studying general questions of gene function. Results of the proposed experiments can be used to enhance these techniques. Finally, it is thought that insect transposable elements will eventually prove useful in fighting insect pests. An understanding of their basic biology, as will be obtained from these experiments, will be essential for any such effort. Methods: A combination of standard genetic and molecular techniques will be employed. Drosophila will be cultured in the laboratory and screened by phenotype for the genetic variations of interest. Molecular techniques include DNA sequencing and polymerase chain reaction for amplification of specific DNA se-