EXPRESSION OF T CELL RECEPTOR GAMMA AND DELTA CHAINS

MHC限制的，T细胞的抗原特异性识别是介导的 通过CD 3受体复合物与α β 异二聚体。 小鼠和人的胸腺和脾脏含有少量的 T细胞群表达另一种CD 3相关的 γ和δ链的受体。 关于这一点，我们知之甚少。 γ-δ受体的功能作用，也不是其个体发育 与阿尔法-贝塔的关系 人们对这个复杂的地方知之甚少， 显然是有序的事件，调节γ和δ基因 表情 我们最近表明，主要的T细胞成分 在小鼠表皮中，Thy 1 + dEC细胞表达γ-δ 受体的限制剧目，类似于早期胎儿 胸腺 此外，我们已经表明，γ链和δ链RNA 在dEC细胞中的合成可以被ConA调节。 使用Thyl+dEC 作为一个模型系统，我们建议通过（1） 确定Con A诱导更高水平的水平 δ链mRNA和较低水平的γ链mRNA， 同时诱导IL-2的分泌。 (2)确定是否 抗γ-δ受体抗体也可诱导 来自dEC克隆的IL-2诱导γ-δ信息蛋白的变化 表面表达。 (3)确定保护的作用 组织特异性启动子-增强子相关序列 γ和δ基因的转录和诱导。 （四） 分离、表征和克隆蛋白质的基因， 在功能上与γ和δ相关调节因子相互作用， 序列的 (5)表征从功能性的自发转换 dEC中的γ-δ至功能性α-β RNA表达 分身

MHC-restricted, antigen-specific recognition by T cells is mediated by a receptor complex of CD3 associated with the alpha beta heterodimer. The thymus and spleen of mice and man contain a small population of T cells that express an alternative CD3-associated receptor of gamma and delta chains. Little is known of the functional role of the gamma-delta receptor, nor its ontogenic relationship to alpha-beta. Less is known of the complex and apparently ordered events that regulate gamma and delta gene expression. We have recently shown that the major T cell component of the mouse epidermis, Thy1+ dEC cells, express gamma-delta receptors of a restricted repertoire, akin to that of early fetal thymus. Moreover, we have shown that gamma and delta chain RNA synthesis in dEC cells can be modulated by Con A. Using Thyl+dEC as a model system, we propose to extend these observations by (1) Establishing the level at which Con A acts to induce higher levels of delta chain mRNA and lower levels of gamma chain mRNA while simultaneously inducing secretion of IL-2. (2) Determining whether anti-gamma-delta receptor antibodies that also induce secretion of IL-2 from dEC clones induce changes in gamma-delta message, protein and surface expression. (3) Establishing the role of conserved promoter-enhancer associated sequences in tissue-specific transcription and induction of gamma and delta genes. (4) Isolating, characterizing and cloning the genes for proteins that functionally interact with gamma and delta associated regulatory sequences. (5) Characterizing a spontaneous switch from functional gamma-delta to functionally alpha-beta RNA expression in a dEC clone.