COMPARTMENTAL ORGANIZATION OF THE YEAST GOLGI COMPLEX

描述(摘自申请者摘要)：格雷厄姆博士的长期任期 目标是了解细胞是如何组装和维护有序的 组成高尔基复合体的舱室的布置，以及如何 这种结构有助于这个重要细胞器的功能。 具体目标是：(1)继续界定隔间 酵母菌高尔基复合体的结构及其所需的因素 蛋白质在高尔基体室间的转运；(2)破译 定位信号(S)在高尔基酵母驻留蛋白中的表达 复合体，Mnn1蛋白(Mnn1p)；(3)分离表现出 Mnn1p高尔基体定位缺陷；(4)分离基因 编码特定于酵母的分类信息的片段 分泌途径。

DESCRIPTION (adapted from applicant's abstract): Dr. Graham's long term goal is to understand how the cell assembles and maintains the ordered arrangement of compartments that comprise the Golgi complex, and how this structure contributes to the function of this essential organelle. The specific aims are: (1) to continue to define the compartmental organization of the yeast Golgi complex and the factors required for protein transport between Golgi compartments; (2) to decipher the localization signal(s) within a resident protein of the yeast Golgi complex, the Mnn1 protein (Mnn1p); (3) to isolate mutants that exhibit a defect in the Golgi localization of the Mnn1p; and (4) to isolate gene fragments that encode sorting information specific to the yeast secretory pathway.