GNRH AND THE MENSTRUAL CYCLE

描述(改编自申请者的摘要)：远距离 这项建议的目标是了解神经内分泌控制 生殖周期，特别是促性腺激素释放激素在灵长类动物中的作用。 由于测量GnRH释放的技术困难和长时间 在灵长类动物中，周期生殖事件的持续时间很少 有关正常促性腺激素释放激素分泌模式的直接信息 月经周期-可获得的信息是零散的 有争议的。首席调查员小组已经开发出一种 雌性恒河猴促性腺激素释放激素分泌的测定方法 监测脑脊液中促性腺激素释放激素水平的直接变化 取自第三脑室漏斗区域的；慢性的 脑室插管提供长时间的GnRH测量 句号。提出了检测促性腺激素释放激素分泌的五个具体目标 正常排卵月经周期和病理状态的模式。 AIM 1将记录个人月经期间的GnRH分泌 循环并首次提供GnRH的复合配置文件 正常周期。目标2将研究促性腺激素释放激素在周期中期的分泌， 在促性腺激素激增和排卵发生的时候， 评估雌激素和黄体酮信号对正常的 GnRH/LH峰。目标3将研究促性腺激素释放激素在周期结束时的分泌， 在黄体-卵泡过渡过程中考虑其在 重新启动卵泡生长。Aim 4将调查影响 雌激素负反馈对GnRH脉冲式释放的影响。目标5将 应激诱导促性腺激素释放激素分泌的病理生理学研究 下丘脑闭经，黄体周期不足， 以及在诱导多个排卵的治疗周期中。

DESCRIPTION (Adapted from the applicant's abstract): The long-range goal of this proposal is to understand the neuroendocrine control of the reproductive cycle, and specifically the role of GnRH in primates. Because of technical difficulties in measuring GnRH release and the long duration of cyclic reproductive events in primates, there is little direct information on the normal GnRH secretory profile during the menstrual cycle - and the available information is fragmentary and controversial. The principal investigator's group has developed a method for measurement of GnRH secretion in the female rhesus monkey by monitoring direct changes in GnRH levels in cerebrospinal fluid (CSF) obtained from the infundibular region of the third ventricle; chronic intraventricular catheterization provides GnRH measurements of long time periods. Five specific aims are proposed to examine GnRH secretory patterns in normal ovulatory menstrual cycles and in pathologic states. Aim 1 will document GnRH secretion throughout individual menstrual cycles and provide for the first time a composite GnRH profile of the normal cycle. Aim 2 will investigate the GnRH secretion at midcycle, at the time when the gonadotropin surge and ovulation occurs, and evaluate the estradiol and progesterone signals required for a proper GnRH/LH surge. Aim 3 will study GnRH secretion at the end of the cycle, during the luteal-follicular transition to consider its role in the reinitiation of follicular growth. Aim 4 will investigate the impact of estrogen negative feedback on GnRH pulsatile release. Aim 5 will study the pathophysiology of GnRH secretion during "stress"-induced hypothalamic amenorrhea, menstrual cycles with inadequate luteal phases, and during treatment cycles for induction of multiple ovulations.