Functional insights into the roles of enteric glia

本项目旨在回顾我们目前对肠道神经胶质细胞种群的定义，以及我们对它们在小鼠和灵长类结肠中的通信机制的理解。它将使用先进的显微镜和图像分析产生关于肠道胶质细胞生物学的新知识。钙成像和新型生物传感器将测量肠道神经胶质细胞的信号。它将提供对灵长类结肠中神经胶质细胞连接、形态和受体表达的最全面的分析。预期结果包括肠道神经胶质细胞的详细地图及其基本生物学定义。这个项目建立在候选人近年来所做的技术和合作的基础上。这将有助于我们对肠道神经胶质细胞生物学的基本了解。

This project aims to review our current definition of enteric glia populations, and our understanding of their communication mechanisms in the mouse and primate colon. It will generate new knowledge of enteric glia biology using advanced microscopy and image analysis. Calcium imaging and novel biosensors will measure cell signalling in enteric glia. It will provide the most comprehensive analysis of glia connectivity, morphology and receptor expression in the primate colon. Expected outcomes include a detailed map of enteric glia and definitions of their basic biology. This project builds on the techniques and collaborations made by the candidate over recent years. This will benefit our basic understanding of enteric glia biology.