MARINE LIPIDS AND POST PRANDIAL MEDIATORS

海洋石油在血管疾病中的潜在健康益处正在被 深入研究，包括它们对血小板的影响， 内皮、巨噬细胞和脂蛋白（LP）。 也有很大 关注餐后脂血症期间致动脉粥样硬化LP的出现， 海洋脂质中的N-3脂肪酸（FA）对后 餐时脂血症现在已经得到了很好的证实。 然而，还没有研究表明， 定义了LP、内皮和血液的餐后相互作用 元素在人类或如何摄入海洋石油可能会改变这种 流程. 这项研究的目的是利用国家- 最先进的GC/MS方法和规定的试验餐给10 研究男性脂蛋白加工过程中血小板/血管的相互作用 和妇女，以及这些影响是如何改变的急性和慢性 海洋石油的摄入; 20评估N-3 FA和α- 生育酚对餐后加工过程中氧化LP生成的影响 正常和动脉粥样硬化受试者，以及通过 含氧脂质的胆汁输送到胃肠道可以被合并到 新生乳糜微粒，和3）研究脂肪酸的转移， 甾醇、其氧化衍生物和α-生育酚对单核细胞的作用 在摄入海产品或对照油后的餐后脂血期间， 以及海洋油和富含海洋油的LP对人单核细胞的影响 和巨噬细胞胆固醇酯代谢，类花生酸合成和 趋化性 生理学和药理学方法将用于 定义血小板/血管、血管/脂蛋白的重要方面， 人类脂蛋白/单核细胞相互作用，比较男性中的这些事件 和妇女，并确定他们如何受到海洋石油的影响。 通过做 因此，拟议的项目直接解决了体内几个重要的问题， 假设人类动脉粥样硬化形成的现有理论，从而 将更清楚地定义海洋石油的潜在健康益处， 西方社会。

The potential health benefits of marine oils in vascular disease are being intensively investigated, including their effects on platelets, endothelium, macrophages and lipoproteins (LP). There is also great interest in the appearance of atherogenic LP during post-prandial lipemia, and striking effects of the N-3 fatty acids (FA) in marine lipids on post- prandial lipemia are now well-established. However, no studies have yet defined the post-prandial interactions of LP, endothelium and blood elements in humans or how ingestion of marine oils might alter such processes. The object of the proposed research will be to utilize state- of-the-art GC/MS methods and administration of defined test meals to 10 study platelet/vascular interactions during lipoprotein processing in men and women, and how such effects are changed by the acute and chronic ingestion of marine oils; 20 assess the effects of N-3 FA and alpha- tocopherol on the generation of oxidized LP during post-prandial processing by normal and atherosclerotic subjects, as well as the delivery via the bile of oxygenated lipids to the G.I. tract that could be incorporated into nascent chylomicrons, and 3) investigate the transfer of fatty acids, sterols, their oxygenated derivatives, and alpha-tocopherol to monocytes during post-prandial lipemia following ingestion of marine or control oils, and the effects of marine oils and marine-oil enriched LP on human monocyte and macrophage cholesterol ester metabolism, eicosanoid synthesis and chemotaxis. Physiological and pharmacological approaches will be used to define important aspects of platelet/vascular, vascular/lipoprotein, and lipoprotein/monocyte interactions in humans, compare these events in men and women, and determine how they are influenced by marine oils. By doing so, the proposed project directly addresses in vivo several important hypotheses underlying current theories of human atherogenesis, and thereby will define more clearly the potential health benefits of marine oils in Western societies.