SPECIATION OF HDL AND LDL

该项目的目的是利用最近在以下方面取得的重大进展 对血浆脂蛋白结构与功能关系的认识 来描述女性的生物过程，这些过程可能 易患冠状动脉疾病或提供对其的保护。是这样的 通过识别新的风险因素，知识可以导致早期 识别处于高危状态的个人，还可以提供 旨在缓解过程的新治疗策略的基础 有助于动脉粥样硬化的形成，或增强那些具有保护性的。 然而，超速离心法似乎颠覆了 高密度脂蛋白(高密度脂蛋白)，一种新开发的非 变性免疫吸附允许分离七到八个 离散的物种。这些新发现物种的数量分布 将在200名育龄和绝经后妇女中进行测量。 单个物种的功能作用将在以下方面进行研究 将胆固醇酯转移到受体脂蛋白，这是一个步骤 对胆固醇恢复途径至关重要。不充分的转让 高密度脂蛋白的胆固醇酯到低密度脂蛋白(LDL)可能是基础 高密度低密度脂蛋白的现象，在一些研究中与 冠心病。本项目将探索高密度脂蛋白与 物种分布对这一现象的影响。低密度脂蛋白的氧化可能是关键 与动脉硬化性心脏病的发展有关。意义重大 高密度脂蛋白对这一过程的抑制已被证明。的目标 这项研究是对所有负责的高密度脂蛋白物种的鉴定 这种抗氧化作用和它们的作用机制。

The aim of this project is to take advantage of major recent advances in knowledge of the structure-function relationships of plasma lipoproteins to characterize, in women, biological processes that are likely to predispose to coronary disease or to confer protection against it. Such knowledge, by identifying new risk factors, could lead to early identification of individuals at high risk and could also provide the basis for novel strategies of treatment designed to mitigate processes that contribute to atherogenesis, or to enhance those that are protective. Whereas ultracentrifugation appears to derange the native architecture of high density lipoproteins (HDL), a newly developed strategy of non- denaturing immunosorption permits the separation of seven to eight discrete species. Quantitative profiles of these newly discovered species will be measured in two hundred reproductive age and postmenopausal women. Functional roles of the individual species will be studied with respect to the transfer of cholesteryl esters to acceptor lipoproteins, a step critical to the cholesterol retrieval pathway. Deficient transfer of cholesteryl esters to low density lipoproteins (LDL) by HDL may underlie the phenomenon of hyperdense LDL, related in some studies to risk of coronary disease. This project will explore the relationship of HDL species distribution to this phenomenon. Oxidation of LDL may be critical to the development of arteriosclerotic heart disease. Significant inhibition of the process by HDL has been demonstrated. An objective of this study is the identification of all the HDL species responsible for this antioxidant effect and the mechanisms by which they act.