BCL-1 REARRANGEMENT IN CENTROCYTIC LYMPHOMA

中心细胞型淋巴瘤是B细胞淋巴瘤的一种生物学亚型 与11号染色体bcl-1重排和PRAD 1相关 过度表达 本课题的主要目的是：1）研究bcl-1基因的表达， 中心细胞淋巴瘤中重排和易位连接的顺序 以确定染色体位置、易位位点和核苷酸 易位连接的序列;这将包括括号， 从许多中心细胞性淋巴瘤中分离易位连接 用肿瘤基因组DNA文库进行bcl-1重排。 亚克隆 将用于染色体作图和原位 杂交和与杂交位点相邻的断裂点的核苷酸序列， 2）进一步研究bcl-1/PRAD 1的表达， 基因组区域，并确定易位相关的断点， 显示PRAD 1过表达但不显示bcl-1的中心细胞淋巴瘤 重排;这将需要双向染色体步行， 限制性内切酶图谱和核苷酸序列分析， 将bcl-1基因与PRAD 1基因连接起来，开发新的DNA探针 可以检测这个区域的易位断点

Centrocytic lymphoma is a biologically distinct subtype of B-cell lymphoma that has been associated with chromosome 11 bcl-1 rearrangements and PRAD1 overexpression. Objectives of the proposal are: 1) to study the bcl-1 rearrangement and translocation junction in centrocytic lymphoma in order to determine the chromosomal locations, translocation sites and nucleotide sequences of the translocation junction; this will include bracketing and isolating the translocation junction from a number of centrocytic lymphomas with bcl-1 rearrangement using libraries of tumor genomic DNA. Subclones from across the breakpoint will be used for chromosomal mapping and in situ hybridization and the nucleotide sequence of the breakpoint adjacent to the bcl-1 region will be determined, and 2) to further dissect the bcl-1/PRAD1 genomic region and identify translocation-related breakpoints in cases of centrocytic lymphoma that show PRAD1 overexpression but not bcl-1 rearrangements; this will entail bi-directional chromosome walking with restriction enzyme mapping and nucleotide sequence analysis to physically link the bcl-1 region and PRAD1 region, and thus develop new DNA probes that can detect translocation breakpoints in this region.