HEMATOPOIETIC KITS FOR OSTEOCLAST FORMATION

描述：这是一项第二阶段的研究方案，以跟进他们的 无血清培养物对破骨细胞支持作用的初步观察 增殖分化。具体地说，调查人员 建议确定破骨细胞生长所需的特定成分； 确定各因素的最优相关性和浓度(S) 并用化学方法取代含血清或无血清的培养液 已定义的组织培养组件。这些媒体将用于评估 小鼠骨髓来源的TRAP+细胞在体外的发育 含有部分纯化的O-CSF的半固体试验。这些研究将 在华盛顿大学演出，由M·李博士指导。 布朗博士的实验室将测试各种成分，包括 转铁蛋白、胰岛素、胆固醇、其他脂质、降低剂和 痕量金属。

DESCRIPTION: This is a phase II research protocol to follow up their initial observations that serum free media could support osteoclast proliferation and differentiation. Specifically, the investigators propose to identify specific components required for osteoclast growth; determine the optimal correlation and concentration of each factor(s) and replace the serum containing or serum free media with chemically defined tissue culture components. These media will be used to assess the development of TRAP+ cells derived from murine bone marrow in a semisolid assay containing partially purified O-CSF. These studies will be performed at Washington University under the direction of Dr. M. Lee. Dr. Brown's laboratory will test various components including transferrin, insulin, cholesterol, other lipids, reducing agents and trace metals.