BIOCHEMICAL RESPONSE OF OSTEOBLASTS TO MATERIAL SURFACES

研究计划将使用细胞培养模型来检查反应 从骨细胞到生物材料。细胞将在电晕上培养- 排放的聚苯乙烯或已溅射涂覆的碟子上 商业纯钛，以评估是否接触钛 改变细胞对生长因子的反应。三个假设将是 测试：(1)细胞增殖和基质合成在以下情况下发生变化 成骨样细胞在钛表面生长；(2)基质囊泡和基质 当这些细胞在钛上培养时，钙化发生改变； 重组人对基质合成和钙化的调控 转化生长因子-β1(TGFβ)在细胞 用钛培养。为了测试这些假设的有效性，以下是 目的是：(1)比较细胞增殖和基质 常规培养的胎鼠颅骨(FRC)细胞的合成 电晕放电聚苯乙烯(塑料)诉镀钛表面；(2)至 表征基质泡囊结构和功能及基质 塑料和钛涂层表面培养的FRC细胞的钙化；以及 (3)确定钛上的培养是否改变FRC细胞对 TGFBeta。

The research program will use a cell culture model to examine the response of bone cells to biomaterials. Cells will be cultured on corona- discharged-polystyrene or on dishes which have been sputter-coated with commercially pure titanium in order to assess whether exposure to Ti alters cellular response to growth factors. Three hypotheses will be tested: (1) Cell proliferation and matrix synthesis are altered when osteoblast-like cells are grown on Ti; (2) Matrix vesicle and matrix calcification are altered when these cells are cultured on Ti; and (3) Regulation of matrix synthesis and calcification by recombinant human transforming growth factor-beta1 (TGFbeta) are altered when cells are cultured by Ti. To test the validity of these hypotheses, the following aims are proposed: (1) To compare cellular proliferation and matrix synthesis of fetal rat calvarial (FRC) cells cultured on conventional corona-discharged-polystyrene (plastic) v. ti-coated surfaces; (2) To characterize matrix vesical structure and function and matrix calcification by FRC cells cultured on plastic v. Ti-coated surfaces; and (3) To determine whether culture on Ti alters FRC cell response to TGFbeta.