APICAL COMPLEX ORGANELLES AND PROTEINS OF BABESIA BOVIS

研究建议：牛巴贝斯虫是最重要的寄生虫之一。 世界各地牛的数量。与巴贝斯虫病相关的发病率和死亡率 和其他重要的复合体疾病，如疟疾、球虫病 和泰勒氏症，目前已促使密集努力改善 控制方法不到位。可能的新控制目标是 顶端复合体，是顶端复合体所特有的，并执行 重要的，虽然还不完全了解，在他们的生活中的功能 循环。超微结构证据有力地证明了 寄主细胞入侵中的顶端复合体。此外，抗病毒抗体 几种顶端复合蛋白可以阻断红细胞的侵袭。这有 还有待于对B.bovis进行演示。在拟议的研究中，1)尖端 将分离出复杂的细胞器；2)多克隆和单克隆 产生的顶端复合体和蛋白质的抗体试剂， 分别进行了分析。这些试剂将在未来的实验中用于检测 抗心尖复合体蛋白抗体将抑制的假说 牛黄杆菌裂殖子对红细胞的侵袭。这一战略是专门设计的 目的：快速鉴定在侵袭中起作用的顶端复杂蛋白。 一旦确定，他们在入侵中所扮演角色的分子细节 这一过程可以在长期研究中进行研究。 应聘者：应聘者是完成临床考试的兽医 兽医解剖病理学住院医师的服务部分， 一位兽医博士候选人。这些实验 本提案中描述的内容将构成HIS的具体目标1和2 博士研究。研究结果将为长期发展提供方向。 目的阐明宿主细胞进入宿主细胞的机制。 顶端复合体寄生虫。 环境：兽医微生物学和病理学系 因在分子生物学和免疫学方面的贡献而被认可 兽医传染病，并设有设施，以应付 在这些领域进行当前的技术研究。 牛巴贝斯虫病的合作研究在以下几个领域中占有突出地位 部门项目。主要赞助商具有以下经验： 原生动物和后生动物寄生虫的分子生物学，包括 胞内寄生虫的发育、分离和鉴定 免疫保护性表面蛋白。

Research Proposal: Babesia bovis is one of the most significant parasites of cattle worldwide. Morbidity and mortality associated with babesiosis and other important apicomplexan diseases, such as malaria, coccidiosis and theileriosis, has prompted intensive efforts to improve currently inadequate control methods. Possible novel targets for control are the apical complex organelles that are unique to the Apicomplexa and perform important, although incompletely understood, functions in their life cycles. Ultrastructural evidence strongly implicates the involvement of the apical complex in host cell invasion. In addition, antibody to several apical complex proteins can block erythrocyte invasion. This has yet to be demonstrated for B. bovis. In the proposed studies, 1) apical complex organelles will be isolated, and 2) polyclonal and monoclonal antibody reagents to apical complex organelles and proteins generated, respectively. These reagents will be used in future experiments to test the hypothesis that antibody to apical complex proteins will inhibit erythrocyte invasion by B. bovis merozoites. This strategy is designed to rapidly identify apical complex proteins which function in invasion. Once identified, the molecular details of their roles in the invasion process can be investigated in long-term studies. Candidate: The candidate is a veterinarian completing the clinical service component of a residency in veterinary anatomic pathology, and is a candidate for the Ph.D. in veterinary science . The experiments described in this proposal will constitute Specific Aims 1 and 2 of her doctoral research. The results will provide directions for the long-term objective of elucidating the mechanisms of host cell entry by apicomplexan parasites. Environment: The Department of Veterinary Microbiology and Pathology is recognized for contributions to the molecular biology and immunology of veterinary infectious diseases, and has facilities equipped for performing technologically current research in these areas. Collaborative research in bovine babesiosis figures prominently among departmental projects. The primary sponsor has experience in the molecular biology of protozoan and metazoan parasites, including intracellular parasite development, and isolation and characterization of immunoprotective surface proteins.