CADHERIN EXPRESSION IN ZEBRAFISH WITH RETROGNATHIC JAW

我的项目是在动物模型中研究颌骨发育。系统 我们将与一组斑马鱼(Brachydanio Rerio)突变体合作。 由于摄食障碍，这些鱼的寿命会缩短 让人想起患有皮埃尔-罗宾综合征的新生儿。 斑马鱼是发育研究的一个很好的模型系统，因为 我们可以比较已经知道的关于正常发育的 斑马鱼和这些变种人。一种推测是细胞-细胞 黏附分子，特别是钙粘附素，对 斑马鱼的形态发生与其他生物的形态发生一样 已经研究过了。 钙粘附素是一个依赖于钙离子的蛋白家族，它是 对胚胎发育至关重要。目前，至少有24名 不同的钙粘附素已经被描述。最具特点的是 它们是E-钙粘蛋白(上皮钙粘蛋白)、N-钙粘蛋白(神经钙粘蛋白)。 P-钙粘素(胎盘钙粘蛋白)和L-CAM(活细胞黏附) 分子)。蛋白质中最保守的区域是细胞质 域。 我们这个项目的第一个目标是开发研究钙粘附素的试剂 在斑马鱼发育过程中的表达。用于免疫组织学和 生物化学研究我们正在制造针对保守分子的抗体 钙粘附素的细胞质区域。用于原位杂交研究 我们根据基因的保守区设计了寡核苷酸引物。 钙粘附素。我们将利用这些引物来获得斑马鱼钙粘附素 CDNA.一旦我们确认了这些试剂的特性，我们就 将使用它们来比较钙粘素在发育过程中的表达 正常的和变异的鱼。 关键词：钙粘附素、斑马鱼、颌后下巴

My project is to study jaw development in an animal model. The system we will work with in a group of zebrafish (Brachydanio rerio) mutants. These fish have a shortened life span due to feeding disabilities reminiscent of newborns with Pierre-Robin Syndrome. Zebrafish are an excellent model system for developmental studies since we can compare what is already known about the normal development of zebrafish with these mutants. One speculation is that cell-cell adhesion molecules, in particular cadherins, are important for morphogenesis in the zebrafish as in other organisms where they have been studied. The cadherins are a family of CA2+- dependent proteins which are essential for embryonic development. Currently, at least two dozen different cadherins have been described. The best characterized of these are E-cadherin (epithelial cadherin), N-cadherin (neural cadherin) P-cadherin (placental cadherin), and L-CAM (live cell adhesion molecule). The most conserved region of the proteins is the cytoplasmic domain. Our first goal in this project is to develop reagents to study cadherin expression during zebrafish development. For immunohistological and biochemical studies we are making antibodies against the conserved cytoplasmic region of the cadherins. For in situ hybridization studies we have made oligonucleotide primers based on the conserved region of the cadherins. We will use these primers to obtain zebrafish cadherin cDNA. Once we have confirmed the specificities of these reagents, we will use them to compare cadherin expression during development of the normal and mutant fish. KEYWORDS: cadherins, zebrafish, retrognathic lower jaw