CYTOKINES IN CHRONIC ADULT PERIDONTITIS

成人牙周炎是一种慢性炎症性疾病，涉及 牙齿的支撑结构。毒力之间的相互作用 牙菌斑中微生物的致病因素与宿主免疫 反应是这种疾病的主要诱因。多种炎性细胞因子 与疾病过程有牵连，尽管直接证据是 目前是有限的。革兰氏阴性杆菌的脂多糖 细菌可能调节牙周局部炎症反应 并与牙周的慢性性质有关 感染，连接组织逐渐破坏，以及 牙槽骨。 由于牙周沟液中含有细胞分泌的产物 在牙龈中，我们开发了确定存在和水平的方法 几种炎性和抗炎细胞因子在样品中的表达 收集自临床健康和牙周病网站。通过 Western印迹检测到肿瘤坏死的细胞因子蛋白 肿瘤坏死因子-α、白介素6与转化生长 在80 ng、40 ng和10 ng的灵敏度水平下的因子-β(TGF-β)， 分别进行了分析。我们的研究涉及收集发炎的 包括牙周组织和细胞因子基因表达的检测 细胞因子信使RNA的反向扩增 转录酶-聚合酶链式反应。我们确认了 细胞因子IL-1α、L-1β、肿瘤坏死因子-α、IL-6和IL-8的信息 在这些组织中。 不同脂多糖对单个核细胞体外反应的检测 然后进行准备工作，培养单核细胞系， U937与不同剂量的来自卟啉单胞菌的内毒素或 大肠埃希氏菌与增殖、细胞活力和炎症 检测细胞因子基因表达。我们的结果表明 浓度和时间依赖的两种作用的差异 细胞上的脂多糖制剂。

Adult periodontitis is a chronic inflammatory disease involving the supporting structures of the teeth. The interaction between virulence factors of microbial pathogens in dental plaque and the host immune response is a major inducer of the disease. Many inflammatory cytokines have been implicated in the disease process, although direct evidence is presently limited. The lipopolysaccharide (LPS) from gram-negative bacteria may regulate the local inflammatory response in the periodontal pocket and be associated with the chronic nature of the periodontal infection, the gradual destruction of connection tissue, and loss of alveolar bone. Since the gingival crevicular fluid contains products secreted by cells in the gingiva, we developed methods to determine the presence and level of several inflammatory and anti-inflammatory cytokins in samples collected from clinically health and periodontally diseased sites. By Western blot we detected the cytokine proteins tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and transforming growth factor-beta (TGF-beta) at sensitivity levels of 80 ng, 40 ng, and 10 ng, respectively. Our studies involving the collection of inflamed periodontal tissues and examination of cytokine gene expression included amplification of cytokine messenger RNA by the reverse transcriptase-polymerase chain reaction. We identified the presence of the message for cytokines IL-1alpha, l-1beta, TNF-alpha, IL-6, and IL-8 in these tissues. Examination of the in vitro response of mononuclear cells to various LPS preparations was then undertaken, Cultures of the monocytic cell line, U937, were incubated with various amounts of LPS from Porphyromonas or Escherichia coli and proliferation, cell viability, and inflammatory cytokine gene expression were assessed. Our results indicate both concentration and time dependent differences in the effects of the two LPS preparations on the cells.