PHOSPHOLAMBAN AND HYPOTHYROID AND HYPERTHYROID HEARTS

我在Fogarty奖学金下的研究目标是澄清 磷蛋白对不同类型甲状腺肌浆网钙泵的调节作用 状态(甲状腺功能低下、甲状腺功能正常和甲状腺功能亢进)。这些研究将 提供适当的方法来确定 磷蛋白对甲状腺心脏SR功能的调节作用及其机制 参与调节这些心脏对β-受体的改变反应- 肾上腺素能刺激。这样的研究可能会为 甲状腺疾病治疗更趋合理的探讨 实验。 在匈牙利的阿尔伯特·森特-乔尔吉医科大学，我在 与临床心脏病专家合作，计划启动生化 心肌病心肌的研究。因此，各种不同的 可用于研究SR功能的生化技术 体外和体内条件(SR制备、表征、 磷蛋白的纯化，SR膜小泡的重建， 放射免疫法测定钙摄取和钙-三磷酸腺苷酶活性 技术、心脏灌流等)对我来说是极其重要的。 在克拉尼亚斯教授在辛辛那提的实验室里，我有很好的机会 掌握所有这些方法，因为所有必要的设备都是 可用和优秀的专家可以帮助我熟悉所有 相关技术。

My research goal under the Fogarty Fellowship would be to clarify the regulatory role of phospholamban on the SR Ca2+-pump in various thyroid states (hypothyroid, euthyroid and hyperthyroid). These studies will provide an appropriate approach to the determination of the role of phospholamban in regulation of the SR function in thyroid hearts and its involvement in mediating the altered response of these hearts to Beta- adrenergic stimulation. Such studies may provide the basis for an approach to the treatment of thyroid disease with more rational experiments. At Albert Szent-Gyorgyi Medical University in Hungary I work in cooperation with a clinical cardiologist and plan to start biochemical studies on cardiomyopathic heart muscle. Accordingly, the various biochemical techniques with which the SR function can be studied under in vitro and in vivo conditions (SR preparation, characterization, purificatioin of phospholamban, reconstitution of SR membrane vesicles, measurement of Ca2+ uptake and Ca2+-ATPase activity, radioimmunoassay techniques, heart perfusion, etc.) would be extremely important for me. At Prof. Kranias's laboratory in Cincinnati, I have a very good chance to master all of these methods, since all of the necessary equipment is available and good experts can help me to become familiar with all of the relevant techniques.