ENTAMOEBA CYST FORMATION

拟议的实验将探索内阿米巴对包囊的反应。 细胞超微结构、基因水平的诱导条件 表达和蛋白质合成。了解要求和 包膜细胞的反应将提供有助于 设计方法来中断寄生虫生命周期的这一阶段， 从而减少该寄生虫的感染形式。 人类人口。E入侵者最初将被用作模型系统 对于组织裂殖吸虫。将优化包囊形成条件，以 从滋养体体外培养获得高产量的包囊。电子 显微镜将用来检查超微结构的变化 沉淀法。新的蛋白质合成将通过新陈代谢进行检测 识别包囊特异性的标记包囊培养物和抗血清 产品。在包囊过程中表达的基因将被分离和使用 监测寄生虫包囊过程中的转录反应。 将寻求支持包囊化的条件， 目的：建立人寄生虫体外包囊系统。

The proposed experiments will explore the response of Entamoeba to cyst- inducing conditions at the levels of cellular ultrastructure, gene expression and protein synthesis. Knowledge of the requirements and responses of the encysting cell will provide information useful for designing approaches to interrupt this stage of the parasite life cycle, thereby lowering the number of infective forms of the parasite in the human population. E. invadens will initially be used as a model system for E. histolytica. Conditions of cyst formation will be optimized to obtain high yields of cysts from in vitro trophozoite cultures. Electron microscopy will be used to examine ultrastructural changes during encystation. Novel protein synthesis will be examined with metabolically labelled encysting cultures and antisera which recognize cyst-specific products. Genes expressed during encystation will be isolated and used to monitor the transcriptional response of parasites during encystment. Conditions which support encystment of E. histolytica will be sought, in order to develop an in vitro cyst-forming system for the human parasite.