MOTILE FUNCTION OF NEUTROPHILS AND CYTOPLASTS

这是一项体内趋化(定向运动)的研究，目的是 了解血中性粒细胞(PMN)寻找途径的过程 到发炎/感染的区域。它使用了独特的趋化作用 Marcel Bessis教授的系统，其中一个红细胞被摧毁 通过激光微照射产生趋化梯度，向上 PMN迁移。该系统允许零时间(激光闪光)，并且 直接观察取向、粘接性能、轨迹、 以及场中每个细胞的相互作用。除此之外，还有一流的 光学、必要时光线增强、实时视频录制或 灰度级或假彩色的时间推移和计算机辅助分析 关于单个或一组细胞的行为。也受雇于这个 系统是有核的、可移动的、颗粒稀少的碎片(细胞质)。 派生自PMN，表示要在其中学习的简化系统 趋化性。目前的工作是在PMN和PMN中解决 细胞质，如对化学趋化性的影响 抑制自由基气体一氧化氮(N)的生成，效果如何 被定义的基材，如涂有重要的 细胞外基质蛋白、纤维连接蛋白和玻璃体连接蛋白及其作用 [Ca~(2+)]i在中性粒细胞和胞质分离能力中的瞬变 从底物开始，因此沿着梯度向上进行。 总而言之，这些研究应该会教会我们很多关于 趋化性的要求；细胞外基质的可能方式 蛋白质可能有助于炎性细胞的招募；以及如何 一氧化氮，一种目前引起强烈兴趣的自由基气体，可能会在 这些事件。

This is an in vivo study of chemotaxis (directed locomotion), aimed at understanding the process by which blood neutrophils (PMN) find their way to areas of inflammation/infection. It employs the unique chemotactic system of Professor Marcel Bessis, in which a red blood cell destroyed by laser microirradiation gives rise to a chemotactic gradient, up which PMN migrate. The system allows for a zero time (the laser flash), and direct observation of the orientation, adhesive properties, trajectories, and interactions of every cell in the field. To this is added superb optics, light enhancement when desired, video recording in real time or time lapse, in gray scale or pseudocolor, and computer-assisted analysis of the behavior of individual or groups of cells. Also employed in this system are a nucleate, motile, granule-poor fragments (cytoplasts) derived from PMN, which represent simplified systems in which to study chemotaxis. The present work is addressing, in PMN and in PMN cytoplasts, such problems as the effects on chemotaxis of agents that inhibit the generation of the radical gas nitric oxide (N)), the effects of defined substrates such as glass coated with the important extracellular matrix proteins, fibronectin and vitronectin, and the role of [Ca2+]i transients in the ability of PMN and of cytoplasts to detach from substrate and therefore proceed up the gradient. In the aggregate, these studies should teach us a great deal about the requirements for chemotaxis; possible ways in which extracellular matrix proteins may help in the recruitment of inflammatory cells; and how nitric oxide, a radical gas of intense current interest, may figure in these events.