PITUITARY GONADAL FUNCTION IN FASTING

一个为期2年的临床研究和培训计划，在大学的 弗吉尼亚综合临床研究中心将由至少两个 关于禁食相关机制的独立研究领域 男性和女性促性腺激素减退症。 研究和培训计划 将由约翰内斯·D·Veldhuis（内政部） 医学/内分泌学和代谢学系，密歇根大学 Virginia，Charlottesville，VA）。 在第一项研究（第一年）中， 促性腺激素释放激素（GnRH）脉冲发生器的能力， 避免空腹促性腺功能减退将在正常体重下进行测试 男人 此外，内源性阿片肽的作用将是 在相同的背景下进行评估。 在第二项研究中（第一年和第二年）， 我将检验禁食会增加负反馈的假设 性类固醇对性腺轴的作用，同时放大它们的 对生长激素（GH）轴的正反馈效应， 绝经后妇女。 这些研究可能会引起对其他研究的后续评估。 假设，如禁食影响女性性腺轴， 月经周期的具体阶段（第二年）。 分析 空腹时促性腺激素（LH和FSH）和GH分泌模式 使用计算机辅助多参数反褶积进行 分析. 这种新颖的生物物理，计算机辅助技术提供了 有机会计算频率，质量，振幅和持续时间 这些激素分泌事件以及估计受试者特异性 激素清除率。 检查分泌模式 在短期禁食期间，男性和女性的促性腺激素应提供 了解饮食的病理生理学的重要线索， 运动引起的人类生殖轴异常。

A 2-year clinical research and training program in the University of Virginia General Clinical Research Center will consist of at least two separate research areas concerning mechanisms of fasting-associated hypogonadotropism in men and women. The research and training program will be supervised by Dr. Johannes D. Veldhuis (Department of Internal Medicine/Division of Endocrinology and Metabolism, University of Virginia, Charlottesville,VA). In the first study (1st year), the ability of a gonadotropin-releasing hormone (GnRH) pulse generator to avert the hypogonadotropism of fasting will be tested in normal weight men. In additions, the role of endogenous opiate peptides will be evaluated in the same context. In the second study (1st and 2nd year), I will test the hypothesis that fasting increases the negative feedback actions of sex steroids on the gonadal axis, while amplifying their positive feedback effects on the growth hormone (GH) axis, in postmenopausal women. These studies may elicit later evaluation of other hypotheses, such as that fasting affects the female gonadal axis in a stage-of-the-menstrual cycle specific manner (2nd year). Analysis of gonadotropin (LH and FSH) and GH secretory patterns during fasting will be carried out using computer-assisted multiple-parameter deconvolution analysis. This novel biophysical, computer-assisted technique gives the opportunity to calculate the frequency, mass, amplitude, and duration of these hormone secretory episodes as well as estimate subject-specific hormone clearance rates. Examining the secretory pattern of gonadotropins during short-term fasting in men and women should provide important clues to understanding the pathophysiology of diet- and exercise-induced abnormalities in the human reproductive axis.