FERAL MOUSE BREEDING COLONY

目的：用生物（激素、逆转录病毒）诱导乳腺肿瘤 穿梭载体和小鼠乳腺肿瘤病毒，MMTV）和化学 致癌物质在各种野生品系的小家鼠和其他物种的 转基因小家鼠品系的培育 激活的原癌基因乳腺癌前病变的维持 通过在同基因小鼠中移植来产生增生的生长系。 方法：保持一个封闭的纯种群体，1,000野生和近交 小鼠殖民地由大约700只老鼠组成， 长期（2年）的肿瘤发展和300只小鼠作为育种核心。 繁殖核心由三个纯种远交群体组成， 具有与研究相关的独特特征的野生小鼠 小鼠乳腺肿瘤的发生。 它们是：CZECHII V-小鼠（Mus musculus musculus）、CzechII V+、MS（M. spretus）小鼠。五个转基因小鼠品系 含有MMTVLTR-Int 3、WAP-Int 3、MMTVLTR-Wnt-1、MMTVLTR-TGF b和 MT-TGF α转基因也得到了维持。TGF α“敲除”小鼠 菌株最近也被引入殖民地。另外还有按 高发病率的C3 H/OuJ，GR/imr， BALB/cfCZECH Ⅱ和BALB/cfMS近交系小鼠的低发病率 保持BALB/cp和FVB近交系小鼠品系。

Goal: Induction of mammary tumors with biological (hormones, retroviral shuttle vectors, and mouse mammary tumor virus, MMTV) and chemical carcinogens in various feral strains of Mus musculus and other species of Mus. Breeding of transgenic strains of Mus musculus containing certain activated protooncogenes. Maintenance of preneoplastic mammary hyperplastic outgrowth lines by transplantation in syngeneic mice. Approach: Maintain a closed pedigreed colony of 1,000 feral and inbred mice. The colony is composed of approximately 700 mice that are held long term (2 years) for tumor development and 300 mice as a breeding nucleus. The breeding nucleus is composed of three pedigreed outbred colonies of feral mice having unique characteristics that are pertinent to the study of mouse mammary tumorigenesis. They are: CZECHII V-mice (Mus musculus musculus), CZECHII V+, MS (M. spretus) mice. Five transgenic mouse lines containing the MMTVLTR-Int3, WAP-Int3, MMTVLTR-Wnt-1, MMTVLTR-TGFb, and MT-TGFa transgenes are also being maintained. The TGFa "knockout" mouse strain has also recently been introduced into the colony. In addition, a limited breeding nucleus of the high-incidence C3H/OuJ, GR/imr, BALB/cfCZECHII, and BALB/cfMS inbred mouse strains and the low-incidence BALB/cp and FVB inbred mouse strains are maintained.