Development of an optimised pancreatic cell tool kit to enable accelerated diabetes drug discovery

开发优化的胰腺细胞工具包，以加速糖尿病药物的发现。糖尿病药物的开发受到缺乏人胰岛及其供体依赖的可变性的限制。我们将从人类诱导的多能干细胞(HiPSCs)中建立用于2型糖尿病研究的胰岛β细胞模型，提高其成熟度和功能。还将生成互补的CRISPR基因编辑的疾病模型。然后，将使用自动化兼容的96或384多孔板格式开发3D细胞培养系统，以实现高通量筛选和下游药物评估。该平台的市场准备情况将由行业测试者进行评估。

Development of an optimised pancreatic cell tool kit to enable accelerated diabetes drug discovery. Diabetes drug discovery is constrained by the lack of availability of human pancreatic islets and their donor-dependent variability. We will develop pancreatic beta cell models for type 2 diabetes research from human induced pluripotent stem cells (hiPSCs), improving their maturity and function. Complementary CRISPR gene-edited disease models will also be generated. 3D cell culture systems will then be developed using automation compatible 96- or384-multiwell plate formats for high throughput screening and downstream drug evaluation. Market readiness of this platform will be evaluated by an industry beta testers.