SEX STEROID AND V VULNIFICUS ENDOTOXIC SCHOCK

描述（改编自研究者摘要）：这是一个学术 研究增强奖提案旨在研究性激素在人类免疫系统中的作用 内毒素性休克 细菌，创伤弧菌。 假设将被测试与拟议的 实验（编号如下）是，不同的激素配置文件之间 男性和女性倾向于男性大大增强敏感性， 创伤弧菌LPS的致命后遗症 这个假设将是 在具体目标1中测试，通过实验（1）测试剂量依赖性 雄性与雌性大鼠对创伤弧菌LPS的反应;在特定目标中 2，实验（2）确定卵巢切除术（ov-x）是否导致增强的 雌性大鼠对LPS的敏感性，以及实验（3）确定是否 睾酮进一步增加LPS诱导的ov-x效应的敏感性 雌性大鼠;在具体目标3，实验（4）中，如果给予去势雄性大鼠 雌二醇-17 β降低了对LPS的反应性; 目的4、实验（5）测定一氧化氮（NO）是否改变 产生改变了大鼠对创伤弧菌LPS的反应性。

DESCRIPTION (Adapted from the Investigator's Abstract): This is an Academic Research Enhancement Award proposal to study the role of sex hormones in the endotoxic shock induced by lipopolysaccharides (LPS) derived from the bacterium, V. vulnificus. The hypothesis to be tested with the proposed experiments (numbered below) is that different hormonal profiles between males and females predisposes the males to greatly enhanced sensitivity to the virulent sequelae of the V. vulnificus LPS. This postulate will be tested in Specific Aim 1, by experiment (1) testing the dose-dependent responses of male versus female rats to V. vulnificus LPS; in Specific Aim 2, experiment (2) determining whether ovariectomy (ov-x) results in enhanced sensitivity of female rats to LPS, and experiment (3) determining if testosterone further increases sensitivity of LPS-induced effects on ov-x female rats; in Specific Aim 3, experiment (4) if castrated male rats given estradiol-17beta have a decrease in responsiveness to LPS; and in Specific Aim 4, experiment (5) determining whether alteration of nitric oxide production shifts the responsiveness of the rats to V. vulnificus LPS.