BETA-ADRENERGIC RECEPTOR MEDIATED PHOSPHORYLATION

我们目前对涎腺腺泡正常功能的认识 细胞及其在口腔健康和疾病中的作用得到了改善 显著然而，精确的分子事件-参与了 唾液腺分泌、生长和发育的调节仍然是 不太了解。近年来，β-肾上腺素能受体 已显示介导的事件在唾液中起重要作用， 腺体细胞生物学，包括细胞生长和分化，能量 代谢、离子转运、蛋白质合成、基因表达、DNA 合成和胞吐作用。更好地理解实际的分子 参与β-肾上腺素能介导的正常细胞调节的事件 唾液腺腺泡细胞的功能将提供必要的 基本信息，以更全面地了解正常的分子和 这些细胞的细胞生物学，以及可能的分子基础， 发病机制见于各种口腔外分泌疾病。 这个研究项目的长期目标是开发新的基础 关于实际分子事件的科学信息， 直接控制在大鼠腮腺和下颌下腺泡细胞内 肾上腺素能受体激活后 我们目前的研究目标是更充分地研究一个积分 膜磷蛋白（pp 26），似乎直接参与 β-肾上腺素能受体介导的信号转导途径 β-肾上腺素能受体刺激后。具体目标包括 大鼠腮腺和下颌下pp 26磷蛋白的序列测定 使用当前的蛋白质化学-蛋白质测序技术， 分子生物学-PCR技术。一旦测序了两个pp 26， 基于计算机的共有序列分析和免疫细胞定位 将进行研究以确定pp 26在 β-肾上腺素能受体介导的刺激。最后，Western blotting 和核糖体探针分析将用于确定组织的范围 pp 26 mRNA的表达和这些磷酸化蛋白的分布， 各种器官。 这些研究应该提供重要的新的科学信息 关于β-肾上腺素能受体 受体刺激及其在正常唾液腺细胞和 分子生物学

Our current understanding of the normal function of salivary gland acinar cells and their role in oral health and disease has improved significantly. However, the precise molecular events -involved in the regulation of salivary gland secretion, growth and development are still poorly understood. Over recent years, the beta-adrenergic receptor mediated events have been shown to play an important role in salivary gland cellular biology, including cell growth and differentiation, energy metabolism, ion transport, protein synthesis, gene expression, DNA synthesis and exocytosis. A better understanding of the actual molecular events involved in beta-adrenergic mediated cellular regulation of normal salivary gland acinar cell function would provide the necessary fundamental information to more fully comprehend the normal molecular and cellular biology of these cells, and the possible molecular basis for the pathogenesis seen in various exocrine diseases of the oral cavity. The long range objective of this research project is to develop new basic scientific information regarding the actual molecular events that are directly controlled within the rat parotid and submandibular acinar cell following beta-adrenergic receptor activation. Our current research objectives are to study more fully an integral membrane phosphoprotein (pp26) which appears to be directly involved in the beta-adrenergic receptor mediated signal transduction pathway(s) following beta-adrenergic receptor stimulation. Specific aims include the sequencing of both rat parotid and submandibular pp26 phosphoprotein using current protein chemistry-protein sequencing techniques and molecular biology-PCR techniques. Once having sequenced both pp26s, computer-based consensus sequence analysis, and immunocytolocalization studies will be done to determine the biological role of pp26 during beta-adrenergic receptor mediated stimulation. Finally, Western blotting and riboprobe analysis will be utilized to determine the extent of tissue expression of pp26 mRNA and distribution of these phosphoproteins in various organs. These studies should provide important new scientific information regarding the intracellular events that occur following beta-adrenergic receptor stimulation and their role in normal salivary gland cellular and molecular biology.