WOUND CONTRACTION--MYOSIN LIGHT CHAIN PHOSPHORYLATION

在儿科外科有一个长期的观察，一个新生儿 很少表现出伤口愈合问题。成人的康复通过一种 精心策划的事件序列，其中新的细胞种群和 新的基质沉积取代了缺陷，这是一个不完美的过程 由于无法恢复正常真皮的全部抗张强度。 通过该方法修复开放的全厚度软组织缺损 伤口收缩，其中成纤维细胞与胶原纤维相互作用 产生收缩力量的。与伤口收缩形成对比的是 疤痕痉挛的并发症，由此引起的收缩过程 发生在愈合的上皮化伤口中，并导致减少 机械功能。在分子水平上，肌动蛋白- 成纤维细胞微丝中的肌球蛋白细丝已被证明 成为推动收缩过程的“发动机”。肌动蛋白-肌球蛋白 相互作用依赖于肌球蛋白轻链的磷酸化 肌球蛋白分子的末端。肌球蛋白光的磷酸化 肌球蛋白轻链激酶(MLCK)需要链(LC_(20)) 激活细胞微丝收缩。MLCK的活性 控制微丝的收缩。通过对这一问题的研究， 胎儿和成人成纤维细胞的分子相互作用，该项目的 具体目的是证明肌球蛋白ATPase的活性 与伤口收缩程度成正比。长的- 学期目标与这个项目的临床意义是平行的。这个 在开放的缺损处控制收缩的能力 改善目前对大型开放性伤口和烧伤的管理。

There is a long-standing observation in pediatric surgery that a neonate seldom demonstrates wound healing problems. Adult healing occurs by an orchestrated sequence of events in which both new cellular population and new matrix deposition replace a defect, an imperfect process exemplified by the inability to regain the full tensile strength of normal dermis. An open full-thickness soft tissue defect is repaired through the process of wound contraction, in which fibroblasts interact with collagen fibrils producing contractile forces. In contrast to wound contraction is the complication of scar contracture, whereby the process of contraction occurs in healed epithelialized wounds and results in decreased mechanical function. At a molecular level, the interaction of actin- myosin filaments in the microfilaments of fibroblasts has been shown to be the "engine" that drives the contractile process. Actin-myosin interaction depends on the phosphorylation of the myosin light chains at the terminal end of the myosin molecule. Phosphorylation of myosin light chains (LC20) by myosin light chain kinase (MLCK) is required for activation of cell microfilament contraction. The activity of MLCK controls the contraction of microfilaments. Through the study of the molecular interactions of fetal and adult fibroblasts, this project's specific aim is to demonstrate that the amount of myosin ATPase activity is directly proportional to the degree of wound contraction. The long- term goals parallel the clinical implications of this project. The ability to control the amount of contraction in an open defect wound improve current management of large open wounds and burns.