HSP-90 AND STEROID RECEPTORS

热休克蛋白90是一种普遍存在的热休克蛋白， 就其对压力的反应以及与类固醇的关系而言， 受体和其他蛋白质。 它的功能仍然未知。 很长的- 这些研究的长期目标是确定 hsp90。 这将通过hsp90的突变作图来实现。 与其他细胞成分相互作用的区域。 一系列HSP 90 已经制备了缺失突变体，并且这些突变体在无细胞系统中。 两 HSP90突变体的其它新测试将在Aim 1中开发。 的 第一个涉及更普遍的hsp90复合物，其中两个涉及萤火虫 荧光素酶复性。 突变体测试的第三阶段将是 在目标2中进行。 这将包括对热休克蛋白90二聚化的测试， ATP的结合和水解，以及hsp90与另一个相关的 蛋白，p59或FKBP 59。 在Aim 3中，将获得一系列新的hsp90突变体。 根据从目标1和2获得的信息，编写并测试了一份报告。 这些突变体将获得精确的突变体， 相互作用或功能与最小的结构变化， 分子。 这些突变体将被用来描述单独的和 HSP90上相互关联的功能结构域，对HSP90的免疫功能至关重要。 生物活动。

Hsp90 is a ubiquitous heat shock protein that has been described mainly in terms of its response to stress an its association with steroid receptors and other proteins. Its function remains unknown. The long- term objective of these studies is to identify functional elements of hsp90. This will be approached through mutational mapping of hsp90 domains of interaction with other cellular components. A series of hsp90 deletion mutants has been prepared and these in a cell-free system. Two additional novel tests for hsp90 mutants will be developed in Aim 1. The first concerns a more general complex of hsp90 with two involving firefly luciferase renaturation. A third phase of mutant testing will be conducted in Aim 2. This will include tests on hsp90 dimerization, the binding and hydrolysis of ATP, and hsp90 binding to another associated protein, p59 or FKBP59. In Aim 3, a new series of hsp90 mutants will be prepared and tested, based on the information gained from Aims 1 and 2. These mutants will be to obtain precise mutant that lack a specific interaction or function with a minimum of structural alteration in the molecule. These mutants will be used to describe separate and interrelated functional domains on hsp90 that are important to its biological activities.