ALTERED GAP-43 AS A FUNCTION OF CONTEXTUAL LEARNING

GAP-43是一种神经元特异性的膜相关蛋白， 在海马体中表达。 它在神经突生长中很重要， 再生和可塑性。 它的调制也与 海马中LTP的诱导和持续性。 GAP-43是一种 蛋白激酶C（PKC）底物，因此可能发挥重要作用， 在学习和记忆过程中的作用是有据可查的， 底物PKC的作用和过程，它改变了学习， 发生的事情还没有被阐明。 该提案旨在探讨 GAP-43在情境恐惧条件反射后的调节， 将表达或磷酸化的任何变化与 学习。 重点将是GAP-43水平的差异， 磷酸化状态作为训练的结果而产生。 有 GAP-43蛋白水平变化的初步证据。 等 GAP-43水平或磷酸化状态的变化可能 调节海马神经递质的释放， 学习发生的机制。 拟议的研究将在 使用蛋白质印迹、磷酸化测定和 神经递质释放测定。

GAP-43 is a neuronal specific, membrane-associated protein highly expressed in the hippocampus. It is important in neurite outgrowth, regeneration and plasticity. Its modulation has also been linked to LTP induction and persistence in the hippocampus. GAP-43 is a protein kinase C (PKC) substrate and as such may play an important role in learning and memory processes is well documented, the substrates PKC acts upon and the processes it alters for learning to occur have not been elucidated. The proposal is designed to explore the modulation of GAP-43 following contextual fear conditioning and correlate any changes in expression or phosphorylation with the degree of learning. Emphasis will be differences in GAP-43 levels and phosphorylationstate arising as a result of training. There is preliminary evidence for changes in GAP-43 protein levels. Such changes in the levels or phosphorylation state of GAP-43 may modulate hippocampal neurotransmitter release, potentially a mechanism for learning to occur. The proposed studies will be carried out using western blotting, phosphorylation assays and neurotransmitter release assays.