AXON GUIDANCE IN THE DROSOPHILA VISUAL SYSTEM

这是一个香农奖提供部分支持的研究 项目不属于指定机构的资助范围，但 在优秀的边缘。香农奖旨在提供 支持测试方法的可行性;支持进一步测试 和完善研究技术;进行二次分析或提供 数据集;或进行可以证明PI的离散项目 研究能力或为已经优秀的 应用程序.以下摘要摘自原始文件 由主要经销商提交。 描述（改编自申请人的摘要）： 拟议的研究是寻找轴突生长所需的基因， 使用诱变方法的寻路和目标识别。 的 动物模型是果蝇幼虫的视神经， 上述现象的简单模型。 视神经跟随着 一种在细胞连接桥上的定型路径，具有两个区域 生长锥在那里暂停然后改变方向。 使用 化学诱变筛选已经分离出许多突变体， 在沿着其路径的各个点影响视神经。 的 分子和遗传特征的突变之一， 摘要（ABS）是提案的核心。 后续研究 所提出的将（1）试图表征ABS以获得见解 分析其生化功能;（2）分析表型损失， 在胚胎中和在胚胎发育过程中， 形成椎间盘，以揭示其功能;（3）进行 ABS的遗传分析，因为它与其他基因相互作用，以确定其 在改变的蛋白质的更复杂背景中的作用。

THIS IS A SHANNON AWARD PROVIDING PARTIAL SUPPORT FOR THE RESEARCH PROJECTS THAT FALL SHORT OF THE ASSIGNED INSTITUTE'S FUNDING RANGE BUT ARE IN THE MARGIN OF EXCELLENCE. THE SHANNON AWARD IS INTENDED TO PROVIDE SUPPORT TO TEST THE FEASIBILITY OF THE APPROACH; DEVELOP FURTHER TESTS AND REFINE RESEARCH TECHNIQUES; PERFORM SECONDARY ANALYSIS OR AVAILABLE DATA SETS; OR CONDUCT DISCRETE PROJECTS THAT CAN DEMONSTRATE THE PI'S RESEARCH CAPABILITIES OR LEND ADDITIONAL WEIGHT TO AN ALREADY MERITORIOUS APPLICATION. THE ABSTRACT BELOW IS TAKEN FROM THE ORIGINAL DOCUMENT SUBMITTED BY THE PRINCIPAL INVESTIGATOR. DESCRIPTION (Adapted from applicant's abstract): The goal of the proposed research is to find genes required for axonal growth, pathfinding and target recognition using a mutagenetic approach. The animal model is the Drosophila optic nerve of the larva which provides a simple model of the aforementioned phenomena. The optic nerve follows a stereotyped path over a connecting bridge of cells and has two regions where growth cones pause and then make directional changes. Using a chemical mutagenesis screen a number of mutants have been isolated that effect the optic nerve at various points along its pathway. The molecular and genetic characterization of one of the mutations called abstract (abs) forms the heart of the proposal. The follow up studies that are proposed will (1) attempt to characterize abs to gain insights into its biochemical function; (2) analyze the phenotype of loss of function or gain of function mutations in the embryo and during imaginal disc formation in hopes of revealing its function; and (3) undertake a genetic analysis of abs as it interacts with other genes to define its role within a more complex background of altered proteins.