AMINO ACID TRANSPORT AFTER SMALL BOWEL RESECTION

描述：(改编自调查人员摘要)这项研究是 旨在促进对活性氨基酸变化的理解 大量小肠切除后肠上皮细胞转运。这个 激素和特定营养素在这些变化中的中介作用 还将接受检查。需要检验的假设是：1.积极的 肠道细胞的氨基酸运输在适应过程中发生了变化。 在大量的肠道切除后。2.特定激素影响氨基酸 在这种适应过程中的酸运输。3.特定营养素， 包括氨基酸和短链脂肪酸(SCFA) 通过提高氨基酸摄取，对切除后有益。 新西兰兔接受70%空回肠切除或假手术。 一周、一个月和六个月后，小肠粘膜将 加工成刷边界膜小泡。摄取谷氨酰胺(Gln)， 精氨酸、丙氨酸和亮氨酸将用快速 混合/过滤技术。血浆神经降压素水平的变化 (NT)表皮生长因子(EGF)和生长激素(GH) 用放射免疫测定法测定。静脉输注NT、EGF、GH和 生长抑素类似物奥曲肽将评估激素对氨基酸的影响 酸运输。奥曲肽的作用将通过免疫进一步验证。 用抗NT、EGF和GH的特异性抗血清中和。膳食 操作包括提供等氮、等热量的饮食 以蛋白质为全蛋白、寡肽或游离氨基酸。SCFA 将以游离丁酸钠或果胶的形式添加。影响 胃肠外注射谷氨酰胺对肠腔和肠壁氨基酸转运的影响 内脏循环也将被量化。最终目标是 开发营养/激素组合，以提高适应能力。它 希望所获得的知识将有助于新的治疗方法 短肠综合征患者的治疗策略。

DESCRIPTION: (Adapted from investigator's abstract) This research is designed to advance understanding of changes in active amino acid transport by the enterocyte after massive small bowel resection. The role of hormones and specific nutrients as mediators of these-changes will also be examined. The hypotheses to be tested are: 1. Active amino acid transport by the enterocyte is altered during adaptation after massive bowel resection. 2. Specific hormones influence amino acid transport during this adaptation. 3. Specific nutrients, including amino acids and short chain fatty acids (SCFA) may be beneficial after resection by improving amino acid uptake. New Zealand rabbits undergo 70% jejunoileal resection or sham operation. One week, one month, and six months later, small bowel mucosa will be processed to brush border membrane vesicles. Uptake of glutamine (GLN), arginine, alanine, and leucine will be quantified using a rapid mixing/filtration technique. Changes in plasma levels of neurotensin (NT) epidermal growth factor (EGF), and growth hormone (GH) will be determined by radioimmunoassay. Infusions of NT, EGF, GH and the somatostatin analogue octreotide will assess hormonal influence on amino acid transport. Octreotide's effect will be further verified by immuno- neutralization with specific antisera to NT, EGF, and GH. Dietary manipulation includes the provision of isonitrogenous, isocaloric diets with protein as whole protein, oligopeptides or free amino acids. SCFA will be supplemented either as free sodium butyrate or pectin. Influence of parenteral GLN on amino acid transport from both the lumen and the splanchnic circulation will also be quantified. The eventual goal is to develop nutrient/hormone combinations that will improve adaptation. It is hoped that the knowledge gained will contribute to new therapeutic strategies for patients with short gut syndrome.