ELECTROPHYSIOLOGY OF CANNABINOID ACTION ON RAT BEHAVIORS

这项研究的目的是阐明神经元的基本功能。 大麻滥用的中枢效应的潜在机制， 特别强调与时间有关的神经过程， 感觉-运动整合，以及学习和记忆过程。 一 大量的证据表明大麻 扰乱运动和时间反应，以及学习和记忆 人类的能力。 使用新开发的技术，20-47个单 现在可以同时记录不同的神经元尖峰序列， 在各种行为任务中大鼠的大脑区域。 内侧 前额叶皮质、纹状体和黑质网状部 (NTR)根据神经解剖学和 行为证据表明这三个区域包含高密度的 大麻素受体，并在运动和 研究任务所需的认知功能。 大鼠将 训练执行延迟匹配/不匹配样本（DMTS/DNMTS）， 时间反应分化（TRD）、反应时间（RT）和 跑步机运动（TM）任务。 DMTS的任务是 旨在估计短期内涉及的认知过程， 记忆和空间辨别功能。 TRD将检查 动物估计主观时间流逝的能力， 做出相应的运动反应。 RT时间表测试了 迅速从鼻镜上退出以响应触发音。 TM 是结合听觉音调和跑步机测试联系的任务 感觉提示（音调）和运动功能的起始之间的关系。 此外，本发明还提供了一种方法， 不同剂量大麻素对自发运动的影响 活动（从活动过度到僵硬，对不同剂量的反应 大麻素）将进行研究。 剂量增加 δ-9-四氢大麻酚（δ-9-THC）， 将测试大麻（0.5mg至10 mg/kg i. p.）。 调查 将决定神经元活动和行为表现的变化 因为δ-9-四氢大麻酚中毒 数据分析包括计算 行为时间事件周围的光栅和事件周围直方图。 神经元之间的相关和同步活动 同一地区和不同地区。 许多人的包围活动 与行为事件有关的神经元将使用 判别分析 这项研究的目的是测试 假设大麻素作用于选择性地破坏成分 皮质-纹状体系统内的信号。

This research proposal is aimed at elucidating the fundamental neuronal mechanism(s) underlying the central effects of marijuana abuse with special emphasis on the neuronal processes involved in timing, sensory-motor integration, and learning and memory process. A substantial amount of evidence has indicated marijuana profoundly disrupts locomotor and timing response, as well as learning and memory ability in human. Using a newly developed technique, 20-47 single neuronal spike trains can now be recorded concurrently in different brain areas of rats during a variety of behavior tasks. The medial prefrontal cortex (MPF), striatum and substantia nigra pars reticulate (NTR) are selected for investigation based on the neuroanatomical and behavioral evidence that these three areas contain a high density of cannabinoid receptor and are critically involved in locomotor and cognitive functions required for the tasks studied. Rats will be trained to perform delayed match/non-match to sample (DMTS/DNMTS), temporal response differentiation (TRD), reaction time (RT), and auditory-cued treadmill locomotion (TM) tasks. DMTS is the task designed to estimate the cognitive procedures involved in short term memory and spatial discrimination functions. TRD will examine the capability of the animal to estimate the passage of subjective time and to make a motor response accordingly. The RT schedule tests the ability of promptly withdrawing from nosepiece in response to trigger tone. TM is the task combining auditory tone and treadmill to test the linkage between a sensory cue (tone) and onset locomotor function. In addition, the effect of different doses of cannabinoid on spontaneous locomotor activity (from hyper-activity to catalepsy in response to different does of cannabinoid) will be investigated. An increasing dose of delta-9-tetrahydrocannabinol (delta-9-THC), the active component of marijuana,will be tested (0.5 mg to 10 mg/kg i.p.). The investigation will determine changes of neuronal activity and behavioral performance under delta-9-THC intoxication. Data analyses include computation of raster and perievent histrograms around behavioral time events. Correlationed and synchronized activities between neurons within the same region and across different regions. Ensemble activity of many neurons in relation to behavioral events will be examined using discriminant analysis. The goal of this research is to test the hypothesis that cannabinoids act to selectively disrupt component signals within the cortico-striatal system.