RABPHILIN3A AND EXOCYTOSIS AND CHROMAFFIN CELLS

描述：激素和神经递质的胞吐释放 细胞间通讯和突触传递的中枢 对内分泌、心血管和心脏的正常功能至关重要 神经系统。该应用程序的长期目标是理解 调控胞吐作用的生化和生理机制。 具体地说，这项申请将阐明RabPhilin3a在 路径。Rab GTP酶参与分泌物的囊泡运输 和内吞途径。RabPhilin3a在大脑中被确认为可能的 Rab3a的效应器。调查人员最近发现，RabPhilin3a是 Rab3a在肾上腺嗜铬细胞中的表达及其对钙离子的促进作用 依赖的分泌物。这项提案的重点是调查 RabPhilin3a在调节胞吐中的作用机制。这个 提案将：1)阐明RabPhilin3a增强 牛肾上腺嗜铬细胞胞吐作用的生物化学和生物化学研究 分泌物的电生理测量。2)确定关系 RabPhilin3a及其突变体的亚细胞定位及其在体内的作用 分泌物。3)研究可能存在的RabPhilin3a途径的新成员 包括内收。将使用各种技术来研究 RabPhilin3a在嗜铬细胞中的功能包括瞬时表达 分泌物和分泌物的生化研究方法 来自单细胞研究的电生理/微安培技术。

DESCRIPTION: Exocytotic release of hormones and neurotransmitters is central to intercellular communication and synaptic transmission and is fundamental to the normal function of the endocrine, cardiovascular and nervous systems. The long-term goal of the application is to understand the biochemical and physiological mechanisms underlying regulated exocytosis. Specifically, this application will elucidate the role of rabphilin3a in the pathway. Rab GTPases are involved in vesicular trafficking in the secretory and endocytotic pathways. Rabphilin3a was identified in brain as a possible effector for rab3a. The investigators recently found that rabphilin3a is expressed with rab3a in adrenal chromaffin cells and enhances calcium dependent secretion. The focus of this proposal is to investigate the mechanisms by which rabphilin3a functions in regulated exocytosis. The proposal will: 1) elucidate the mechanism by which rabphilin3a enhances exocytosis in bovine adrenal chromaffin cells using both biochemical and electrophysiological measures of secretion. 2) Determine the relationship between subcellular localization of rabphilin3a and mutants and function in secretion. 3) Investigate possible new members of the rabphilin3a pathway including adducin. A variety of techniques will be used to study rabphilin3a function in chromaffin cells including transient-expression approach for biochemical studies of secretion and electrophysiological/microamperometric techniques from single cell studies.