NEW BIOMEDICAL INTERFACE MATERIALS

用于心血管假体的生物材料遭受众所周知的 与表面诱导的血栓形成和感染相关的问题。 这些界面问题是仍然存在的主要问题， 尚未解决并限制了现有技术的成功和适用性 生物医学聚合物。 拟议的研究将继续侧重于编写新的 通过新型表面改性的生物医学界面材料 旨在改进东道国对现有 生物材料植入物具体来说，将使用界面材料 研究熵排斥水化力（ERF）的作用 血液相容性。将要检验的假设是， 是吸附的主要决定因素， 吸附血浆的组成、组织和功能状态 蛋白质，然后决定随后的细胞和分子表面 交互.我们建议在临床相关性上检验假设 生物材料和控制，表面改性的一系列接口 材料的范围从疏水性到亲水性， 水合度，通过射频等离子体聚合制备， 到将用于最大化ERF的高度溶剂化的寡糖， 由新的寡糖-烃-寡糖（OHO）制成， 三嵌段生物聚合物，将结合到测试表面， 疏水相互作用每种表面改性都将表征 通过光谱和物理方法，包括原子力 原子力显微镜（AFM），这将允许在亚分子水平上成像 以及分子间力的研究。的稳定性 界面材料将在暴露于水、缓冲液 和SDS溶液在动态流动条件下。的影响 血浆蛋白和血小板表面界面特性及电流变液 将在体外研究相互作用， 和动态流动条件，（l）量、组成和 吸附的关键血浆蛋白的功能完整性 蛋白质溶液、去除粘附蛋白的培养基和人 血浆;使用放射性标记方法，红外光谱，免疫标记 技术采用单克隆抗体粘附蛋白质，并通过亚分子AFM 蛋白质成像;和（2）的量和功能状态 吸附血小板 从这些研究中，我们将试图确定的机制， 界面性质和ERF的改变影响了 吸附和功能状态的粘附蛋白，以及如何这 与血液相容性有关。研究建议，如果 成功，将导致重要的新类的发展， 生物医学界面材料，表现出对蛋白质的抵抗力， 吸附作用。

Biomaterials used in cardiovascular prostheses suffer from well-known problems associated with surface-induced thrombosis and infection. These interfacial problems are major concerns that have remained unresolved and limited the success and applicability of existing biomedical polymers. The proposed studies will continue to focus on the preparation of new biomedical interface materials, through novel surface modification methodologies, designed to improve the host response to existing biomaterial implants. Specifically, interface materials will be used to investigate of the role of entropic repulsive hydration forces (ERF) on blood compatibility. The hypothesis that will be tested is that ERF represents a major determining factor in the adsorption, and the composition, organization and functional state of adsorbed plasma proteins, which then determines subsequent cell and molecular surface interactions. We propose to test the hypothesis on clinically-relevant biomaterials and controls, surface-modified with a series of interface materials that range from hydrophobic, to hydrophilic with increasing degree of hydration, prepared by radiofrequency plasma polymerization, to highly solvated oligosaccharides that will used to maximize ERF, prepared from novel oligosaccharide-hydrocarbon-oligosaccharide (OHO) triblock biopolymers that will be bound to test surfaces by strong hydrophobic interaction. Each surface modification will characterized by spectroscopic and physical methods, including atomic force microscopy (AFM) which will permit imaging at the submolecular level and examination of the intermolecular forces. The stability of the interface materials will be determined after exposure to water, buffer and SDS solution, under dynamic flow conditions. The effect of interfacial properties and ERF on plasma protein and platelet-surface interactions will be investigated in vitro by determining, under static and dynamic flow conditions, (l) the amount, composition, and functional integrity of key plasma proteins adsorbed from protein~solutions, media depleted of adhesive proteins, and human plasma; using radiolabel methods, infrared spectroscopy, immunolabel techniques employing Mab to adhesive proteins, and by submolecular AFM imaging of proteins; and (2) the amount and functional state of adsorbed platelets. From these studies, we shall attempt to determine the mechanism by which alteration in interfacial properties and ERF affects the adsorption and functional state of adhesive proteins, and how this correlates to blood compatibility. The proposed research, if successful, will lead to the development of important new classes of biomedical interface materials, that exhibit resistance to protein adsorption.